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Title of the item:

An efficient approach to identify different chemical markers between fibrous root and rhizome of Anemarrhena asphodeloides by ultra high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry with multivariate statistical analysis.

Title :
An efficient approach to identify different chemical markers between fibrous root and rhizome of Anemarrhena asphodeloides by ultra high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry with multivariate statistical analysis.
Authors :
Wang FX; Beijing Institute of Radiation Medicine, Beijing 100850, PR China; Tianjin University of Traditional Chinese Medicine, Tianjin 300193, PR China.
Yuan JC; Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
Kang LP; Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
Pang X; Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
Yan RY; Ovation Health Science and Technology Co. Ltd., ENN Group, Langfang 065001, PR China.
Zhao Y; Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
Zhang J; Ovation Health Science and Technology Co. Ltd., ENN Group, Langfang 065001, PR China.
Sun XG; Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
Ma BP; Beijing Institute of Radiation Medicine, Beijing 100850, PR China. Electronic address: .
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Source :
Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2016 Sep 10; Vol. 129, pp. 105-116. Date of Electronic Publication: 2016 Jun 23.
Publication Type :
Journal Article
Language :
English
Imprint Name(s) :
Publication: <2006->: London : Elsevier Science
Original Publication: Oxford ; New York : Pergamon Press, c1983-
MeSH Terms :
Anemarrhena/*chemistry
Plant Extracts/*analysis
Plant Extracts/*chemistry
Plant Roots/*chemistry
Rhizome/*chemistry
Tandem Mass Spectrometry/*methods
Chromatography, High Pressure Liquid/methods ; Chromatography, High Pressure Liquid/standards ; Multivariate Analysis ; Tandem Mass Spectrometry/standards
Contributed Indexing :
Keywords: Anemarrhena asphodeloides; Multivariate statistical analysis; Steroidal saponins; Structure elucidation; UHPLC-Q-TOF MS(E)
Substance Nomenclature :
0 (Plant Extracts)
Entry Date(s) :
Date Created: 20160715 Date Completed: 20170512 Latest Revision: 20170808
Update Code :
20210914
DOI :
10.1016/j.jpba.2016.06.038
PMID :
27416524
Academic Journal
An ultra high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry approach coupled with multivariate statistical analysis was established and applied to rapidly distinguish the chemical differences between fibrous root and rhizome of Anemarrhena asphodeloides. The datasets of tR-m/z pairs, ion intensity and sample code were processed by principal component analysis and orthogonal partial least squares discriminant analysis. Chemical markers could be identified based on their exact mass data, fragmentation characteristics, and retention times. And the new compounds among chemical markers could be isolated rapidly guided by the ultra high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry and their definitive structures would be further elucidated by NMR spectra. Using this approach, twenty-four markers were identified on line including nine new saponins and five new steroidal saponins of them were obtained in pure form. The study validated this proposed approach as a suitable method for identification of the chemical differences between various medicinal parts in order to expand medicinal parts and increase the utilization rate of resources.
(Copyright © 2016 Elsevier B.V. All rights reserved.)

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