Dynamics of Dengue Virus (DENV)-Specific B Cells in the Response to DENV Serotype 1 Infections, Using Flow Cytometry With Labeled Virions.

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Abstrakt

Tytuł:: Dynamics of Dengue Virus (DENV)-Specific B Cells in the Response to DENV Serotype 1 Infections, Using Flow Cytometry With Labeled Virions.
Autorzy:: Woda M; Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester Institute for Immunology and Informatics, University of Rhode Island, Providence.
Friberg H; Walter Reed Army Institute of Research, Silver Spring, Maryland.
Currier JR; Walter Reed Army Institute of Research, Silver Spring, Maryland.
Srikiatkhachorn A; Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Macareo LR; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Green S; Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester.
Jarman RG; Walter Reed Army Institute of Research, Silver Spring, Maryland.
Rothman AL; Institute for Immunology and Informatics, University of Rhode Island, Providence.
Mathew A; Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester Institute for Immunology and Informatics, University of Rhode Island, Providence.
Źródło:: The Journal of infectious diseases [J Infect Dis] 2016 Oct 01; Vol. 214 (7), pp. 1001-9. Date of Electronic Publication: 2016 Jul 20.
Typ publikacji:: Evaluation Study; Journal Article
Język:: English
Imprint Name(s):: Publication: Jan. 2011- : Oxford : Oxford University Press
Original Publication: 1904-2010 : Chicago, IL : University of Chicago Press
MeSH Terms:: Serogroup*
B-Lymphocytes/*immunology
Dengue/*immunology
Dengue/*virology
Dengue Virus/*immunology
Immunoassay/*methods
Adolescent ; Child ; Child, Preschool ; Female ; Flow Cytometry ; Humans ; Infant ; Male ; Staining and Labeling/methods ; Thailand ; Virion/immunology
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Grant Information:: P01 AI034533 United States AI NIAID NIH HHS; R21 AI113479 United States AI NIAID NIH HHS; U19 AI057319 United States AI NIAID NIH HHS
Contributed Indexing:: Keywords: Alexa Fluor; B cell; Dengue; antigen; flow cytometry
Entry Date(s):: Date Created: 20160723 Date Completed: 20170508 Latest Revision: 20191210
Update Code:: 20240104
PubMed Central ID:: PMC5021233
DOI:: 10.1093/infdis/jiw308
PMID:: 27443614
: Czasopismo naukowe

Background: The development of reagents to identify and characterize antigen-specific B cells has been challenging.
Methods: We recently developed Alexa Fluor-labeled dengue viruses (AF DENVs) to characterize antigen-specific B cells in the peripheral blood of DENV-immune individuals.
Results: In this study, we used AF DENV serotype 1 (AF DENV-1) together with AF DENV-2 on peripheral blood mononuclear cells (PBMCs) from children in Thailand with acute primary or secondary DENV-1 infections to analyze the phenotypes of antigen-specific B cells that reflected their exposure or clinical diagnosis. DENV serotype-specific and cross-reactive B cells were identified in PBMCs from all subjects. Frequencies of AF DENV(+) class-switched memory B cells (IgD(-)CD27(+) CD19(+) cells) reached up to 8% during acute infection and early convalescence. AF DENV-labeled B cells expressed high levels of CD27 and CD38 during acute infection, characteristic of plasmablasts, and transitioned into memory B cells (CD38(-)CD27(+)) at the early convalescent time point. There was higher activation of memory B cells early during acute secondary infection, suggesting reactivation from a previous DENV infection.
Conclusions: AF DENVs reveal changes in the phenotype of DENV serotype-specific and cross-reactive B cells during and after natural DENV infection and could be useful in analysis of the response to DENV vaccination.
(© The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.)

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