Metabolic engineering of cottonseed oil biosynthesis pathway via RNA interference.

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Tytuł:: Metabolic engineering of cottonseed oil biosynthesis pathway via RNA interference.
Autorzy:: Xu Z; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Shizishan Street, Wuhan, Hubei 430070, China.
Li J; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Shizishan Street, Wuhan, Hubei 430070, China.
Guo X; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Shizishan Street, Wuhan, Hubei 430070, China.
Jin S; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Shizishan Street, Wuhan, Hubei 430070, China.
Zhang X; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Shizishan Street, Wuhan, Hubei 430070, China.
Źródło:: Scientific reports [Sci Rep] 2016 Sep 13; Vol. 6, pp. 33342. Date of Electronic Publication: 2016 Sep 13.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:: Biosynthetic Pathways/*genetics
Cottonseed Oil/*chemical synthesis
Metabolic Engineering/*methods
Plants, Genetically Modified/*genetics
Cottonseed Oil/metabolism ; Gene Expression Regulation, Plant ; Gossypium/enzymology ; Gossypium/genetics ; Plants, Genetically Modified/metabolism ; Seeds
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Substance Nomenclature:: 0 (Cottonseed Oil)
Entry Date(s):: Date Created: 20160914 Date Completed: 20180529 Latest Revision: 20240325
Update Code:: 20240325
PubMed Central ID:: PMC5020431
DOI:: 10.1038/srep33342
PMID:: 27620452
: Czasopismo naukowe

Pełny tekst

Cottonseed oil is recognized as an important oil in food industry for its unique characters: low flavor reversion and the high level of antioxidants (VitaminE) as well as unsaturated fatty acid. However, the cottonseed oil content of cultivated cotton (Gossypium hirsutum) is only around 20%. In this study, we modified the accumulation of oils by the down-regulation of phosphoenolpyruvate carboxylase 1 (GhPEPC1) via RNA interference in transgenic cotton plants. The qRT-PCR and enzyme activity assay revealed that the transcription and expression of GhPEPC1 was dramatically down-regulated in transgenic lines. Consequently, the cottonseed oil content in several transgenic lines showed a significant (P < 0.01) increase (up to 16.7%) without obvious phenotypic changes under filed condition when compared to the control plants. In order to elucidate the molecular mechanism of GhPEPC1 in the regulation of seed oil content, we quantified the expression of the carbon metabolism related genes of transgenic GhPEPC1 RNAi lines by transcriptome analysis. This analysis revealed the decrease of GhPEPC1 expression led to the increase expression of triacylglycerol biosynthesis-related genes, which eventually contributed to the lipid biosynthesis in cotton. This result provides a valuable information for cottonseed oil biosynthesis pathway and shows the potential of creating high cottonseed oil germplasm by RNAi strategy for cotton breeding.

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