Euphorbia humifusa Willd exerts inhibition of breast cancer cell invasion and metastasis through inhibition of TNFα-induced MMP-9 expression.

Szczegóły
Abstrakt

Tytuł:: Euphorbia humifusa Willd exerts inhibition of breast cancer cell invasion and metastasis through inhibition of TNFα-induced MMP-9 expression.
Autorzy:: Shin SY; Department of Biological Sciences, College of Biological Science and Biotechnology, Konkuk University, Seoul, Republic of Korea.; Cancer and Metabolism Institute, Konkuk University, Seoul, Republic of Korea.
Kim CG; Cancer and Metabolism Institute, Konkuk University, Seoul, Republic of Korea.
Jung YJ; Cancer and Metabolism Institute, Konkuk University, Seoul, Republic of Korea.
Jung Y; Division of Bioscience and Biotechnology, College of Biological Science and Biotechnology, BMIC, Konkuk University, Seoul, Republic of Korea.
Jung H; Division of Bioscience and Biotechnology, College of Biological Science and Biotechnology, BMIC, Konkuk University, Seoul, Republic of Korea.
Im J; Division of Bioscience and Biotechnology, College of Biological Science and Biotechnology, BMIC, Konkuk University, Seoul, Republic of Korea.
Lim Y; Division of Bioscience and Biotechnology, College of Biological Science and Biotechnology, BMIC, Konkuk University, Seoul, Republic of Korea. .; College of Biological Science and Biotechnology, Konkuk University, 120 Neundong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. .
Lee YH; Department of Biological Sciences, College of Biological Science and Biotechnology, Konkuk University, Seoul, Republic of Korea. .; Cancer and Metabolism Institute, Konkuk University, Seoul, Republic of Korea. .; College of Biological Science and Biotechnology, Konkuk University, 120 Neundong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. .
Źródło:: BMC complementary and alternative medicine [BMC Complement Altern Med] 2016 Oct 24; Vol. 16 (1), pp. 413. Date of Electronic Publication: 2016 Oct 24.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: London : BioMed Central, [2001-2019]
MeSH Terms:: Antineoplastic Agents/*pharmacology
Breast Neoplasms/*metabolism
Euphorbia/*chemistry
Matrix Metalloproteinase 9/*metabolism
Plant Extracts/*pharmacology
Tumor Necrosis Factor-alpha/*metabolism
Animals ; Antineoplastic Agents/chemistry ; Cell Line, Tumor ; Cell Movement/drug effects ; Female ; Humans ; Mice ; Neoplasm Invasiveness ; Plant Extracts/chemistry ; Tumor Cells, Cultured ; Xenograft Model Antitumor Assays
References:: Cancer Res. 1989 Aug 15;49(16):4615-21. (PMID: 2545347)
Nature. 2010 Jan 28;463(7280):485-92. (PMID: 20110992)
Nat Rev Cancer. 2009 Apr;9(4):239-52. (PMID: 19279573)
Genes Dev. 1993 Nov;7(11):2063. (PMID: 8224837)
Cancer Metastasis Rev. 2006 Mar;25(1):9-34. (PMID: 16680569)
Br J Cancer. 2001 Jun 1;84(11):1488-96. (PMID: 11384099)
FEBS Lett. 1998 Sep 11;435(1):29-34. (PMID: 9755853)
Clin Cancer Res. 2012 Sep 1;18(17):4669-81. (PMID: 22806876)
Mol Cancer Res. 2010 Apr;8(4):507-19. (PMID: 20332214)
J Biol Chem. 2010 Oct 1;285(40):30731-40. (PMID: 20650898)
J Natl Cancer Inst. 1986 Dec;77(6):1303-8. (PMID: 3467119)
Phytother Res. 2015 Jul;29(7):1019-25. (PMID: 25919446)
Chem Pharm Bull (Tokyo). 1994 Sep;42(9):1803-7. (PMID: 7954930)
Bioorg Med Chem Lett. 2015 Jan 1;25(1):128-32. (PMID: 25466202)
Biochim Biophys Acta. 2013 Oct;1829(10):1066-74. (PMID: 23872552)
Nat Rev Cancer. 2009 May;9(5):361-71. (PMID: 19343034)
Trends Mol Med. 2002 Aug;8(8):385-9. (PMID: 12127724)
Crit Rev Biochem Mol Biol. 2002 Dec;37(6):375-536. (PMID: 12540195)
Fitoterapia. 2010 Oct;81(7):799-802. (PMID: 20450964)
J Asian Nat Prod Res. 2008 May-Jun;10(5-6):531-9. (PMID: 18470805)
Cancer Res. 1992 Mar 15;52(6):1399-405. (PMID: 1540948)
Fitoterapia. 2011 Mar;82(2):251-4. (PMID: 20940034)
Biochim Biophys Acta. 2010 Jan;1803(1):3-19. (PMID: 19631700)
Bioorg Med Chem Lett. 2014 Apr 15;24(8):1895-900. (PMID: 24679441)
J Clin Invest. 1997 Dec 15;100(12):2952-60. (PMID: 9399940)
Cancer Cell. 2004 Sep;6(3):203-8. (PMID: 15380510)
CA Cancer J Clin. 2011 Mar-Apr;61(2):69-90. (PMID: 21296855)
J Agric Food Chem. 2013 Dec 26;61(51):12588-97. (PMID: 24308485)
J Biol Chem. 2001 Jun 22;276(25):22382-7. (PMID: 11297551)
Nature. 2008 Jul 24;454(7203):436-44. (PMID: 18650914)
J Biol Chem. 2002 May 10;277(19):16639-47. (PMID: 11872748)
J Ethnopharmacol. 2013 Jul 9;148(2):655-63. (PMID: 23707330)
Contributed Indexing:: Keywords: Euphorbia humifusa Willd; Invasion; Matrix metalloproteinase-9; Metastasis; Nuclear factor-kappa B; Tumor necrosis factor alpha
Substance Nomenclature:: 0 (Antineoplastic Agents)
0 (Plant Extracts)
0 (Tumor Necrosis Factor-alpha)
EC 3.4.24.35 (Matrix Metalloproteinase 9)
Entry Date(s):: Date Created: 20161026 Date Completed: 20170221 Latest Revision: 20220408
Update Code:: 20240105
PubMed Central ID:: PMC5078950
DOI:: 10.1186/s12906-016-1404-6
PMID:: 27776550
: Czasopismo naukowe

Full Text Finder

Background: Breast cancer is the most common type of malignancy in women worldwide. Euphorbia humifusa Willd (EuH) is a plant that is widely used as a traditional medicine. However, no systemic studies on the anti-cancer effects of EuH have been reported. The aim of this study is to evaluate the anti-metastatic effect of the EuH.
Methods: Ethyl acetate fraction was prepared from EuH methanol extracts (EA/EuH). Inhibitory effect of EA/EuH on cell migration was determined using an in vitro scratch-wound healing assay. The anti-invasive activity was determined by in vitro three-dimensional spheroid culture system and in vivo syngenic experimental lung metastasis experiment. Gene expression profiles were analyzed by using RT-PCR, real-time PCR, and luciferase reporter assay systems.
Results: Ethyl acetate fraction from the EuH extract (EA/EuH) inhibited the migration and invasive capabilities of highly metastatic MDA-MB-231 breast cancer cells and attenuated syngeneic lung metastasis of mouse 4 T1 breast cancer cells in vivo. Mechanistically, EA/EuH decreased tumor necrosis factor alpha (TNFα)-induced matrix metalloproteinase (MMP)-9 mRNA expression through the inhibition of NF-κB activity in MDA-MB-231 cells.
Conclusion: EuH may be beneficial in the prevention of invasion and metastasis of early stage breast cancer and can be served as an anti-metastatic agent or adjuvant therapy against metastatic breast cancer.

Informacja