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Tytuł:
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Comparative sequence analysis of enteroaggregative Escherichia coli heat-stable enterotoxin 1 identified in Korean and Japanese Escherichia coli strains.
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Autorzy:
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Seo DJ; Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
Choi S; Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
Jeon SB; Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
Jeong S; Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
Park H; Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
Lee BH; Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
Kim GB; School of Bioresources and Bioscience, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
Yang SJ; School of Bioresources and Bioscience, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
Nishikawa Y; Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
Choi C; Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea. Electronic address: .
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Źródło:
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International journal of food microbiology [Int J Food Microbiol] 2017 Feb 21; Vol. 243, pp. 1-8. Date of Electronic Publication: 2016 Nov 21.
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Typ publikacji:
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Comparative Study; Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam : Elsevier Science Publishers, c1984-
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MeSH Terms:
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Enterotoxigenic Escherichia coli/*genetics
Enterotoxigenic Escherichia coli/*isolation & purification
Enterotoxins/*genetics
Escherichia coli Proteins/*genetics
Amino Acid Sequence ; Animals ; Base Sequence ; Cattle ; Electrophoresis, Gel, Pulsed-Field ; Enterotoxigenic Escherichia coli/pathogenicity ; Escherichia coli Infections/microbiology ; Feces/microbiology ; Hot Temperature ; Humans ; Japan ; Meat/microbiology ; Plasmids ; Polymerase Chain Reaction ; Republic of Korea ; Sequence Analysis, DNA ; Swine/microbiology ; Swine Diseases/microbiology ; Virulence/genetics
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Contributed Indexing:
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Keywords: Enteroaggregative Escherichia coli heat-stable enterotoxin 1 (EAST1); Escherichia coli; Pulse field gel-electrophoresis (PFGE); Sequencing; astA
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Substance Nomenclature:
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0 (Enterotoxins)
0 (Escherichia coli Proteins)
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Entry Date(s):
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Date Created: 20161209 Date Completed: 20170407 Latest Revision: 20181202
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Update Code:
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20240105
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DOI:
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10.1016/j.ijfoodmicro.2016.11.017
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PMID:
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27930940
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The aim of this study was to compare the sequence of the astA gene found in 8 Korean and 11 Japanese Escherichia coli isolates. Conventional PCR was used to amplify the astA gene from the chromosomal and plasmid DNA preparation samples of each isolate using commercial DNA extraction kits. Cloning of the PCR products, sequence analysis, and pulse field gel electrophoresis (PFGE) were sequentially performed. An identical copy of astA in each isolate were found for 8 Korean and 8 Japanese E. coli strains isolated from bovine, porcine, and healthy human carriers. Among these, 1 Korean and 4 Japanese isolates carried a stop mutation at residue 16. Three Japanese outbreak strains (V199, V638, and 96-127-23) carried multiple clones of astA gene with multiple amino acids changes at residues 11, 16, 20, 23, 30, 33, and 34. Compared with the non-diarrheal isolates, clonal diversity and sequence variations of the astA gene in outbreak isolates may be associated with virulence potential of EAST1.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
