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Tytuł pozycji:

Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids from Sulfolobus acidocaldarius .

Tytuł :
Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids from Sulfolobus acidocaldarius .
Autorzy :
Engelhardt KH; Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Marburg, Germany.
Pinnapireddy SR; Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Marburg, Germany.
Baghdan E; Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Marburg, Germany.
Jedelská J; Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Marburg, Germany.
Bakowsky U; Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Marburg, Germany.
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Źródło :
Archaea (Vancouver, B.C.) [Archaea] 2017 Jan 23; Vol. 2017, pp. 8047149. Date of Electronic Publication: 2017 Jan 23 (Print Publication: 2017).
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Publication: New York, N.Y. : Hindawi Pub. Corp
Original Publication: Victoria, B.C. : Heron Pub., c2002-
MeSH Terms :
Lipids/*chemistry
Liposomes/*chemistry
Sulfolobus acidocaldarius/*chemistry
Transfection/*methods
Cell Line, Tumor ; Humans
References :
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Substance Nomenclature :
0 (Lipids)
0 (Liposomes)
Entry Date(s) :
Date Created: 20170228 Date Completed: 20171218 Latest Revision: 20181202
Update Code :
20210210
PubMed Central ID :
PMC5292391
DOI :
10.1155/2017/8047149
PMID :
28239294
Czasopismo naukowe
Lipid vectors are commonly used to facilitate the transfer of nucleic acids into mammalian cells. In this study, two fractions of tetraether lipids from the archaea Sulfolobus acidocaldarius were extracted and purified using different methods. The purified lipid fractions polar lipid fraction E (PLFE) and hydrolysed glycerol-dialkyl-nonitol tetraether (hGDNT) differ in their structures, charge, size, and miscibility from conventional lipids. Liposomes were prepared by mixing tetraether lipids with cholesterol (CH) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) resulting in stable vectors for gene delivery. Lipoplexes were prepared by complexation of liposomes with a luciferase expressing plasmid (pCMV-luc) at certain nitrogen-to-phosphorus (N/P) ratios and optimised for the transient transfection of ovarian adenocarcinoma cells (SK-OV-3). Complexation efficacy was investigated by gel-red fluorescence assay. Biophysical properties, like size, surface charge, and morphology, were investigated by differential light scattering (DLS), atomic force microscopy (AFM), and scanning electron microscopy (Cryo-SEM), respectively, revealing structural differences between liposomes and lipoplexes. A range of stable transfecting agents containing tetraether lipids were obtained by incorporating 5 mol% of tetraether lipids. Lipoplexes showed a decrease in free gel-red with increasing N/P ratios indicating efficient incorporation of plasmid DNA (pDNA) and remarkable stability. Transfection experiments of the lipoplexes revealed successful and superior transfection of SK-OV-3 cell line compared to the commercially available DOTAP and branched polyethyleneimine (25 kDa bPEI).

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