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Tytuł:
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Etiological agents of viral meningitis in children from a dengue-endemic area, Southeast region of Brazil.
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Autorzy:
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de Oliveira DB; Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil; Faculdade de Medicina de Diamantina, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina 39100-000, Brazil.
Candiani TM; Hospital Infantil João Paulo II, FHEMIG, Belo Horizonte 30130-110, Brazil.
Franco-Luiz APM; Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Almeida GMF; Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Abrahão JS; Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Rios M; Center for Biologics Research and Review, Food and Drug Administration, Silver Spring, MD 20993, USA.
Coimbra RS; Neurogenômica, Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte 30190-002, Brazil.
Kroon EG; Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil. Electronic address: .
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Źródło:
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Journal of the neurological sciences [J Neurol Sci] 2017 Apr 15; Vol. 375, pp. 390-394. Date of Electronic Publication: 2017 Feb 16.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam : Elsevier, <19 ->
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MeSH Terms:
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Dengue/*epidemiology
Dengue Virus/*pathogenicity
Meningitis, Viral/*epidemiology
Meningitis, Viral/*etiology
Analysis of Variance ; Brazil/epidemiology ; Child ; Child, Preschool ; DNA, Viral/genetics ; DNA, Viral/metabolism ; Dengue/cerebrospinal fluid ; Dengue Virus/genetics ; Dengue Virus/immunology ; Enterovirus/genetics ; Enzyme-Linked Immunosorbent Assay ; Female ; Flavivirus/genetics ; Humans ; Immunoglobulin M/metabolism ; Infant ; Infant, Newborn ; Male ; Meningitis, Viral/cerebrospinal fluid ; Simplexvirus/genetics
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Contributed Indexing:
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Keywords: Dengue virus in CNS; Epidemiology; Molecular diagnosis; Viral meningitis
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Substance Nomenclature:
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0 (DNA, Viral)
0 (Immunoglobulin M)
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Entry Date(s):
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Date Created: 20170322 Date Completed: 20170705 Latest Revision: 20171212
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Update Code:
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20240105
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DOI:
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10.1016/j.jns.2017.02.025
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PMID:
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28320174
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Meningitis is a disease with a global distribution that constitutes a worldwide burden, with viruses as the primary etiologic agents. The range of viral meningitis severity depends mainly on age, immune status and etiological agent. The aim of this work was to investigate the suspected cases of viral meningitis using molecular techniques to confirm the viral infection. The diagnosed virus was correlated with clinical findings and cytochemical parameters in cerebrospinal liquid (CSF) of patients. CSF of 70 children with the presumptive diagnosis of viral meningitis was analyzed by real time PCR (qPCR). Viruses were identified by qPCR in 44 CSF samples (62.9%). Among them, 31 were identified as Enterovirus (ENTV) (70.4%), six as Human herpes virus 3 (HHV-3) (13.6%), five as Dengue virus (DENV) (11.7%), one as Human herpes virus 1-2 (2.3%) and one as Human herpes virus 5 (2.3%). Patients in the HHV-positive groups had increased percentage of polymorphonuclear neutrophils (PMN) (mean of 81%) while the groups of patients with DENV and ENTV had a mean of 30.9%. This study contributes to the knowledge of the epidemiological distribution of viral agents in CNS infections in children. In addition, it raises the relevance of DENV as an agent of CNS infection, and reinforces the importance for molecular in the cases of CNV infection.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
