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Tytuł pozycji:

Oxygenated hypothermic machine perfusion after static cold storage improves endothelial function of extended criteria donor livers.

Tytuł:
Oxygenated hypothermic machine perfusion after static cold storage improves endothelial function of extended criteria donor livers.
Autorzy:
Burlage LC; Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands; Section of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands.
Karimian N; Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands; Section of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands.
Westerkamp AC; Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands; Section of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands.
Visser N; Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands.
Matton APM; Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands; Section of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands.
van Rijn R; Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands; Section of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands.
Adelmeijer J; Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands.
Wiersema-Buist J; Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands.
Gouw ASH; Department of Pathology, University of Groningen, University Medical Center Groningen, The Netherlands.
Lisman T; Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands.
Porte RJ; Section of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University of Groningen, University Medical Center Groningen, The Netherlands. Electronic address: .
Źródło:
HPB : the official journal of the International Hepato Pancreato Biliary Association [HPB (Oxford)] 2017 Jun; Vol. 19 (6), pp. 538-546. Date of Electronic Publication: 2017 Mar 25.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Publication: 2016- : Oxford, UK : Elsevier
Original Publication: 1999-2001: Oxford, England : ISIS Medical Media
MeSH Terms:
Cold Temperature*
Hepatectomy*/adverse effects
Endothelial Cells/*metabolism
Liver/*surgery
Liver Transplantation/*methods
Organ Preservation/*methods
Oxygen/*metabolism
Perfusion/*methods
Tissue Donors/*supply & distribution
Aged ; Cell Survival ; Donor Selection ; Endothelial Cells/pathology ; Female ; Gene Expression Regulation ; Humans ; Kruppel-Like Transcription Factors/genetics ; Kruppel-Like Transcription Factors/metabolism ; Liver/metabolism ; Liver/pathology ; Liver Transplantation/adverse effects ; Male ; Middle Aged ; Netherlands ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type III/genetics ; Nitric Oxide Synthase Type III/metabolism ; Organ Preservation/adverse effects ; Organ Preservation/instrumentation ; Perfusion/adverse effects ; Perfusion/instrumentation ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Thrombomodulin/genetics ; Thrombomodulin/metabolism ; Time Factors
Substance Nomenclature:
0 (KLF2 protein, human)
0 (Kruppel-Like Transcription Factors)
0 (RNA, Messenger)
0 (THBD protein, human)
0 (Thrombomodulin)
31C4KY9ESH (Nitric Oxide)
EC 1.14.13.39 (NOS3 protein, human)
EC 1.14.13.39 (Nitric Oxide Synthase Type III)
S88TT14065 (Oxygen)
Entry Date(s):
Date Created: 20170330 Date Completed: 20180402 Latest Revision: 20220330
Update Code:
20240105
DOI:
10.1016/j.hpb.2017.02.439
PMID:
28351756
Czasopismo naukowe
Background: Lack of oxygen and biomechanical stimulation during static cold storage (SCS) of donor livers compromises endothelial cell function. We investigated the effect of end-ischemic oxygenated hypothermic machine perfusion (HMP) on endothelial cell function of extended criteria donor (ECD) livers.
Methods: Eighteen livers, declined for transplantation, were transported to our center using static cold storage (SCS). After SCS, 6 livers underwent two hours of HMP, and subsequent normothermic machine perfusion (NMP) to assess viability. Twelve control livers underwent NMP immediately after SCS. mRNA expression of transcription factor Krüppel-like-factor 2 (KLF2), endothelial nitric oxide synthase (eNOS), and thrombomodulin (TM) was quantified by RT-PCR. Endothelial cell function and injury were assessed by nitric oxide (NO) production and release of TM into the perfusate.
Results: In HMP livers, mRNA expression of KLF2 (p = 0.043), eNOS (p = 0.028), and TM (p = 0.028) increased significantly during NMP. In parallel, NO levels increased during NMP in HMP livers but not in controls. At the end of NMP cumulative TM release was significantly lower HMP livers, compared to controls (p = 0.028).
Conclusion: A short period of two hours oxygenated HMP restores endothelial cell viability after SCS and subsequent normothermic reoxygenation of ECD livers.
(Copyright © 2017 International Hepato-Pancreato-Biliary Association Inc. Published by Elsevier Ltd. All rights reserved.)

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