-
Tytuł:
-
Immersing lungs in hydrogen-rich saline attenuates lung ischaemia-reperfusion injury.
-
Autorzy:
-
Takahashi M; Central Animal Division, National Cancer Center Research Institute, Chuo-ku, Tokyo, Japan.
Chen-Yoshikawa TF; Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Saito M; Department of Aerospace Psychology, Nagoya University, Japan.
Tanaka S; Research Institute of Disaster management and EMS, Kokushikan University, Tokyo, Japan.
Miyamoto E; Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Ohata K; Department of Gastroenterology, NTT Medical Center Tokyo,Higashi-gotanda, Shinagawa-ku, Japan.
Kondo T; Department of Internal Medicine, Division of Gastroenterology, Hyogo College of Medicine, Hyogo, Japan.
Motoyama H; Department of Hepatology, Osaka City University Graduate School of Medicine, Osaka, Japan
Hijiya K; Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Aoyama A; Department of Public Health and Health Systems, Nagoya University School of Medicine, Nagoya, Japan.
Date H; Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
-
Źródło:
-
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery [Eur J Cardiothorac Surg] 2017 Mar 01; Vol. 51 (3), pp. 442-448.
-
Typ publikacji:
-
Evaluation Study; Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Publication: 2012-: Oxford, England : Oxford University Press
Original Publication: [Berlin] : Springer International ; [Secaucus, NJ, USA : Springer-Verlag New York Inc., distributor, c1987-
-
MeSH Terms:
-
Antioxidants/*pharmacokinetics
Hydrogen/*pharmacokinetics
Lung/*blood supply
Reperfusion Injury/*prevention & control
Animals ; Antioxidants/pharmacology ; Cytokines/biosynthesis ; Hydrogen/pharmacology ; Inflammation Mediators/metabolism ; Lung/metabolism ; Lung Compliance/drug effects ; Organ Preservation ; Organ Preservation Solutions/chemistry ; Organ Preservation Solutions/pharmacology ; Oxygen Consumption/drug effects ; Rats, Inbred Lew ; Reperfusion Injury/metabolism ; Reperfusion Injury/physiopathology ; Sodium Chloride ; Solubility
-
Contributed Indexing:
-
Keywords: Hydrogen-rich solution; Lung ischaemia–reperfusion injury; Lung preservation
-
Substance Nomenclature:
-
0 (Antioxidants)
0 (Cytokines)
0 (Inflammation Mediators)
0 (Organ Preservation Solutions)
451W47IQ8X (Sodium Chloride)
7YNJ3PO35Z (Hydrogen)
-
Entry Date(s):
-
Date Created: 20170402 Date Completed: 20171010 Latest Revision: 20191210
-
Update Code:
-
20240105
-
DOI:
-
10.1093/ejcts/ezw342
-
PMID:
-
28364439
-
Objectives: Anti-oxidant effects of hydrogen have been reported in studies examining ischaemia-reperfusion injury (IRI). In this study, we evaluated the therapeutic efficacy of immersing lungs in hydrogen-rich saline on lung IRI.
Methods: Lewis rats were divided into three groups: (i) sham, (ii) normal saline and (iii) hydrogen-rich saline. In the first experiment, the left thoracic cavity was filled with either normal saline or hydrogen-rich saline for 1 h. Then, we measured the hydrogen concentration in the left lung using a sensor gas chromatograph ( N = 3 per group). In the second experiment, lung IRI was induced by occlusion of the left pulmonary hilum for 1 h, followed by reperfusion for 3 h. During the ischaemic period, the left thoracic cavity was filled with either normal saline or hydrogen-rich saline. After reperfusion, we assessed lung function, histological changes and cytokine production ( N = 5-7 per group).
Results: Immersing lungs in hydrogen-rich saline resulted in an elevated hydrogen concentration in the lung (6.9 ± 2.9 μmol/1 g lung). After IRI, pulmonary function (pulmonary compliance and oxygenation levels) was significantly higher in the hydrogen-rich saline group than in the normal saline group ( P < 0.05). Similarly, pro-inflammatory cytokine levels (interleukin-1β and interleukin-6) in the left lung were significantly lower in the hydrogen-rich saline group than in the normal saline group ( P < 0.05).
Conclusions: Immersing lungs in hydrogen-rich saline delivered hydrogen into the lung and consequently attenuated lung IRI. Hydrogen-rich solution appears to be a promising approach to managing lung IRI.
(© The Author 2016. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.)
