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Tytuł:
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In vitro anti-Candida activity of selective serotonin reuptake inhibitors against fluconazole-resistant strains and their activity against biofilm-forming isolates.
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Autorzy:
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Costa Silva RA; Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil; Department of Pathology and Legal Medicine, School of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil.
da Silva CR; Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil; Christus University Center (UNICHRISTUS), Fortaleza, CE, Brazil.
de Andrade Neto JB; Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil; Department of Pathology and Legal Medicine, School of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil.
da Silva AR; Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.
Campos RS; Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil; Christus University Center (UNICHRISTUS), Fortaleza, CE, Brazil.
Sampaio LS; Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil; Department of Pathology and Legal Medicine, School of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil.
do Nascimento FBSA; Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil; Department of Pathology and Legal Medicine, School of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil.
da Silva Gaspar B; Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.
da Cruz Fonseca SG; Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.
Josino MAA; Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil; Department of Pathology and Legal Medicine, School of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil.
Grangeiro TB; Department of Biology, Science Center, Molecular Genetics Laboratory, Federal University of Ceará, CE, Brazil.
Gaspar DM; Department of Physiology and Pharmacology, Neuropharmacology Laboratory, Federal University of Ceará, Fortaleza, CE, Brazil.
de Lucena DF; Department of Physiology and Pharmacology, Neuropharmacology Laboratory, Federal University of Ceará, Fortaleza, CE, Brazil.
de Moraes MO; Department of Physiology and Pharmacology, Neuropharmacology Laboratory, Federal University of Ceará, Fortaleza, CE, Brazil.
Cavalcanti BC; Department of Physiology and Pharmacology, Neuropharmacology Laboratory, Federal University of Ceará, Fortaleza, CE, Brazil.
Nobre Júnior HV; Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil; Department of Pathology and Legal Medicine, School of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil. Electronic address: label_.
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Źródło:
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Microbial pathogenesis [Microb Pathog] 2017 Jun; Vol. 107, pp. 341-348. Date of Electronic Publication: 2017 Apr 12.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: London ; Orlando : Academic Press, c1986-
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MeSH Terms:
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Antifungal Agents/*pharmacology
Biofilms/*drug effects
Candida/*drug effects
Drug Resistance, Fungal/*drug effects
Fluconazole/*pharmacology
Selective Serotonin Reuptake Inhibitors/*pharmacology
Animals ; Apoptosis/drug effects ; Biofilms/growth & development ; Candida/cytology ; Candida/genetics ; Candida/growth & development ; Cell Count ; Cell Death/drug effects ; Cell Line ; Cell Proliferation/drug effects ; DNA Damage/drug effects ; DNA, Fungal/drug effects ; Fibroblasts/microbiology ; Flow Cytometry ; In Vitro Techniques ; Membrane Potentials ; Mice ; Microbial Sensitivity Tests ; Microbial Viability/drug effects ; Mitochondrial Membranes/drug effects ; Paroxetine/pharmacology ; Plasma/drug effects ; Selective Serotonin Reuptake Inhibitors/administration & dosage ; Sertraline/pharmacology
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Contributed Indexing:
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Keywords: Biofilm; Candida spp.; Fluoxetine; Paroxetine; Sertraline
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Substance Nomenclature:
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0 (Antifungal Agents)
0 (DNA, Fungal)
0 (Serotonin Uptake Inhibitors)
41VRH5220H (Paroxetine)
8VZV102JFY (Fluconazole)
QUC7NX6WMB (Sertraline)
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Entry Date(s):
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Date Created: 20170416 Date Completed: 20171116 Latest Revision: 20221207
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Update Code:
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20240105
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DOI:
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10.1016/j.micpath.2017.04.008
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PMID:
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28411060
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Recent research has shown broad antifungal activity of the classic antidepressants selective serotonin reuptake inhibitors (SSRIs). This fact, combined with the increased cross-resistance frequency of the genre Candida regarding the main treatment today, fluconazole, requires the development of novel therapeutic strategies. In that context, this study aimed to assess the antifungal potential of fluoxetine, sertraline, and paroxetine against fluconazole-resistant Candida spp. planktonic cells, as well as to assess the mechanism of action and the viability of biofilms treated with fluoxetine. After 24 h, the fluconazole-resistant Candida spp. strains showed minimum inhibitory concentration (MIC) in the ranges of 20-160 μg/mL for fluoxetine, 10-20 μg/mL for sertraline, and 10-100.8 μg/mL for paroxetine by the broth microdilution method (M27-A3). According to our data by flow cytometry, each of the SSRIs cause fungal death after damaging the plasma and mitochondrial membrane, which activates apoptotic signaling pathways and leads to dose-dependant cell viability loss. Regarding biofilm-forming isolates, the fluoxetine reduce mature biofilm of all the species tested. Therefore, it is concluded that SSRIs are capable of inhibit the growth in vitro of Candida spp., both in planktonic form, as biofilm, inducing cellular death by apoptosis.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
