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Tytuł:
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Antibiotics in 16-day-old broilers temporarily affect microbial and immune parameters in the gut.
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Autorzy:
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Wisselink HJ; Wageningen Bioveterinary Research, P.O. Box 65, 8200 AB Lelystad, The Netherlands. Electronic address: .
Cornelissen JBWJ; Wageningen Bioveterinary Research, P.O. Box 65, 8200 AB Lelystad, The Netherlands.
Mevius DJ; Wageningen Bioveterinary Research, P.O. Box 65, 8200 AB Lelystad, The Netherlands; Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine, Yalelaan 1, 3584 CL Utrecht, The Netherlands.
Smits MA; Wageningen Bioveterinary Research, P.O. Box 65, 8200 AB Lelystad, The Netherlands; Wageningen Livestock Research, P.O. Box 338, 6700 AH Wageningen, The Netherlands.
Smidt H; Laboratory of Microbiology, Wageningen University & Research, P.O. Box 8033, 6700EH Wageningen, The Netherlands.
Rebel JMJ; Wageningen Livestock Research, P.O. Box 338, 6700 AH Wageningen, The Netherlands.
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Źródło:
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Poultry science [Poult Sci] 2017 Sep 01; Vol. 96 (9), pp. 3068-3078.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: 2020- : [Cambridge, UK] : Elsevier
Original Publication: Champaign Il : Poultry Science Association
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MeSH Terms:
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Amoxicillin/*pharmacology
Anti-Bacterial Agents/*pharmacology
Chickens/*immunology
Chickens/*microbiology
Fluoroquinolones/*pharmacology
Gastrointestinal Microbiome/*drug effects
Intestines/*immunology
Age Factors ; Amoxicillin/administration & dosage ; Animals ; Anti-Bacterial Agents/administration & dosage ; Bacteria/classification ; Enrofloxacin ; Fluoroquinolones/administration & dosage ; Intestines/drug effects ; Male ; Oligonucleotide Array Sequence Analysis/veterinary ; RNA, Bacterial/analysis ; RNA, Ribosomal, 16S/analysis ; Random Allocation ; Real-Time Polymerase Chain Reaction/veterinary
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Contributed Indexing:
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Keywords: antibiotics; chicken; gut; intestinal immunity; microbiota
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Substance Nomenclature:
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0 (Anti-Bacterial Agents)
0 (Fluoroquinolones)
0 (RNA, Bacterial)
0 (RNA, Ribosomal, 16S)
3DX3XEK1BN (Enrofloxacin)
804826J2HU (Amoxicillin)
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Entry Date(s):
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Date Created: 20170609 Date Completed: 20180514 Latest Revision: 20200613
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Update Code:
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20240104
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DOI:
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10.3382/ps/pex133
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PMID:
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28595274
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Animal health benefits from a stable intestinal homeostasis, for which proper development and functioning of the intestinal microbiota and immune system are essential. It has been established that changes in microbial colonization in early life (the first 2 wk post hatch) impacts the functioning of the adult gut and the associated crosstalk between microbiota and intestinal mucosal cells. The aim of the present study was to study the effect of the administration of antibiotics later in life (d 15 to 20 post hatch) on microbiota and immune parameters. For this purpose, chickens received from 15 d post hatch during 5 d amoxicillin or enrofloxacin through their drinking water. Before and at 6, 16, and 27 d after start of the administration of antibiotics, the composition of the microbiota in the jejunum was determined using a 16S ribosomal RNA gene-targeted DNA microarray, the CHICKChip. At 6 d after the start of the administration of the antibiotics, the composition and diversity of the microbiota were affected significantly (P < 0.05), but this change was small and observed only temporarily since differences disappeared at 16 d after initiating treatment with amoxillin and at 27 d after starting treatment with enrofloxacin. Intestinal morphology and development were not visibly affected since there were no differences between villus/crypt ratios and numbers of PAS+ and PCNA+ cells in the duodenum and jejunum at any time point. At 16 d after the start of antibiotic administration, the number of CD4+ T-cells and CD8+ T-cells in the duodenum was lower compared to the control animals; however, this difference was not significant. At some time points, significant differences (P < 0.05) were observed among the groups to locally expressed IL-8, IL-1β, IFN-γ, IL-2, and IL-4 mRNA. However, this effect was not long lasting, as differences that were observed at 16 d after starting the treatment had disappeared at 27 d after treatment was started. The results of this study indicate that later in the broiler's life, antibiotics only temporarily affect intestinal microbial and immune parameters.
(© 2017 Poultry Science Association Inc.)