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Tytuł:
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Lebetimonas natsushimae sp. nov., a novel strictly anaerobic, moderately thermophilic chemoautotroph isolated from a deep-sea hydrothermal vent polychaete nest in the Mid-Okinawa Trough.
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Autorzy:
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Nagata R; Laboratory of Marine Environmental Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Takaki Y; Department of Subsurface Geobiological Analysis and Research (D-SUGAR), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.
Tame A; Department of Technical Services, Marine Works Japan, Ltd., Yokosuka, Japan.
Nunoura T; Marine Functional Biology Group, Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.
Muto H; Laboratory of Marine Environmental Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Mino S; Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan.
Sawayama S; Laboratory of Marine Environmental Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Takai K; Department of Subsurface Geobiological Analysis and Research (D-SUGAR), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.
Nakagawa S; Laboratory of Marine Environmental Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan; Department of Subsurface Geobiological Analysis and Research (D-SUGAR), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan. Electronic address: .
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Źródło:
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Systematic and applied microbiology [Syst Appl Microbiol] 2017 Sep; Vol. 40 (6), pp. 352-356. Date of Electronic Publication: 2017 Jul 04.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: [Munich, Germany] : Elsevier GmbH
Original Publication: Stuttgart ; New York : G. Fischer Verlag, c1983-
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MeSH Terms:
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Water Microbiology*
Epsilonproteobacteria/*classification
Epsilonproteobacteria/*isolation & purification
Hydrothermal Vents/*microbiology
Seawater/*microbiology
Bacteria, Anaerobic ; Base Composition ; Epsilonproteobacteria/genetics ; Epsilonproteobacteria/metabolism ; Fatty Acids/chemistry ; Fatty Acids/metabolism ; Genome, Bacterial ; Phylogeny ; RNA, Ribosomal, 16S/genetics
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Contributed Indexing:
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Keywords: Chemoautotroph; Deep-sea hydrothermal vent; Epsilonproteobacteria; Lebetimonas; Nautiliales; Thermophile
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Substance Nomenclature:
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0 (Fatty Acids)
0 (RNA, Ribosomal, 16S)
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Entry Date(s):
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Date Created: 20170711 Date Completed: 20180503 Latest Revision: 20180503
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Update Code:
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20240104
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DOI:
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10.1016/j.syapm.2017.06.002
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PMID:
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28690052
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A moderately thermophilic, strictly anaerobic, chemoautotrophic bacterium, designated strain HS1857 T , was isolated from a deep-sea hydrothermal vent at the Noho site in the Mid-Okinawa Trough. Strain HS1857 T grew between 35 and 63°C (optimum 55°C), in the presence of 10-55gl -1 NaCl (optimum 25gl -1 ), and pH 5.5-7.1 (optimum 6.4). Growth occurred with molecular hydrogen as the electron donor and elemental sulfur, nitrate, or selenate as the electron acceptors. Formate could serve as an alternative electron donor with nitrate as an electron acceptor. During growth with nitrate as the electron acceptor, strain HS1857 T produced ammonium and formed a biofilm. CO 2 was utilized as the sole carbon source. The G+C content of the genomic DNA was 33.2mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain HS1857 T is a member of the order Nautiliales, showing a sequence similarity of 95.0% with Lebetimonas acidiphila Pd55 T . The fatty acid composition was similar to that of L. acidiphila, which was dominated by C 18:0 (47.0%) and C 18:1 (23.7%). Based on the genomic, chemotaxonomic, phenotypic characteristics, the name Lebetimonas natsushimae sp. nov., is proposed. The type strain is HS1857 T (=NBRC 112478 T =DSM 104102 T ).
(Copyright © 2017 Elsevier GmbH. All rights reserved.)
