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Tytuł:
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Cromolyn chitosan nanoparticles as a novel protective approach for colorectal cancer.
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Autorzy:
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Motawi TK; Biochemistry Department, Faculty of Pharmacy, Cairo University, Kasr El Ainy st., Cairo 11562, Egypt. Electronic address: .
El-Maraghy SA; Biochemistry Department, Faculty of Pharmacy, Cairo University, Kasr El Ainy st., Cairo 11562, Egypt. Electronic address: .
ElMeshad AN; Pharmaceutics and Industrial Pharmacy Department, Faculty of Pharmacy, Cairo University, Kasr El Ainy st., Cairo 11562, Egypt. Electronic address: .
Nady OM; Biochemistry Department, Faculty of Pharmacy, Cairo University, Kasr El Ainy st., Cairo 11562, Egypt. Electronic address: .
Hammam OA; Pathology Department, Theodor Bilharz Research Institute, Warak El-Hadar, Imbaba P.O. Box 30.12411, Giza, Egypt.
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Źródło:
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Chemico-biological interactions [Chem Biol Interact] 2017 Sep 25; Vol. 275, pp. 1-12. Date of Electronic Publication: 2017 Jul 18.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Limerick : Elsevier
Original Publication: Amsterdam, Elsevier.
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MeSH Terms:
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Chitosan/*chemistry
Cromolyn Sodium/*chemistry
Cromolyn Sodium/*pharmacology
Nanoparticles/*chemistry
Animals ; Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/therapeutic use ; Colorectal Neoplasms/chemically induced ; Colorectal Neoplasms/drug therapy ; Colorectal Neoplasms/pathology ; Cromolyn Sodium/therapeutic use ; Dimethylhydrazines/toxicity ; Drug Carriers/chemistry ; Drug Liberation ; Lipid Peroxidation/drug effects ; Male ; Particle Size ; Proto-Oncogene Proteins c-akt/metabolism ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Rats ; Rats, Wistar ; Signal Transduction/drug effects ; beta Catenin/metabolism
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Contributed Indexing:
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Keywords: Chitosan; Colorectal cancer; Cromolyn; Nanoparticles; β-catenin
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Substance Nomenclature:
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0 (Antineoplastic Agents)
0 (Dimethylhydrazines)
0 (Drug Carriers)
0 (Proto-Oncogene Proteins c-bcl-2)
0 (beta Catenin)
9012-76-4 (Chitosan)
EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
Q2WXR1I0PK (Cromolyn Sodium)
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Entry Date(s):
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Date Created: 20170723 Date Completed: 20170926 Latest Revision: 20170926
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Update Code:
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20240104
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DOI:
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10.1016/j.cbi.2017.07.013
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PMID:
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28732690
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Colorectal cancer is the third most common cancer in the world. Cromolyn is a mast cell stabilizer and was proposed as an anticancer agent; however its high polarity limits its bioavailability by rapid washing from the body. We formulated 10 cromolyn chitosan nanoparticles (CCSNPs) 1 following ionic gelation technique to improve its bioavailability and investigated the protective anticancer effect of the optimum formula against colorectal cancer in dimethylhydrazine-induced model in rats. Rats were divided into seven groups, group-1: normal control, group-2: cromolyn control, group-3: CCSNPs control, groups-4 to 7 received dimethylhydrazine for 16 weeks to induce colorectal cancer. Groups-5 to 7 received cromolyn solution, non-medicated chitosan nanoparticles and CCSNPs, respectively as protective treatments. Optimum CCSNPs (size 112.4 nm, charge +39.9 mV, enclosed 93.6% cromolyn and showed a sustained drug release pattern over 48 h) significantly reduced tumor-signaling molecules and the number of aberrant crypt foci compared to dimethylhydrazine. Histopathological examination of colon samples revealed that CCSNPs exerted an augmented protective anticancer effect by ameliorating tumor pathology compared to cromolyn solution. In conclusion, CCSNPs ameliorated tumor pathology and malignant oncogenic signaling molecules in colorectal cancer tissue. Thus, CCSNPs may provide a novel protective approach in colorectal cancer treatment. Moreover, encapsulating cromolyn in chitosan nanoparticles augmented the protective anticancer effect of the drug.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
