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Tytuł:
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Non-invasive assessment of culture media from goat cloned embryos associated with subjective morphology by gas chromatography - mass spectroscopy-based metabolomic analysis.
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Autorzy:
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Zhang YL; Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science, Nanjing Agricultural University, Nanjing, China.
Zhang GM; Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science, Nanjing Agricultural University, Nanjing, China.
Jia RX; Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science, Nanjing Agricultural University, Nanjing, China.
Wan YJ; Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science, Nanjing Agricultural University, Nanjing, China.
Yang H; Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science, Nanjing Agricultural University, Nanjing, China.
Sun LW; Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science, Nanjing Agricultural University, Nanjing, China.
Han L; Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science, Nanjing Agricultural University, Nanjing, China.
Wang F; Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science, Nanjing Agricultural University, Nanjing, China.
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Źródło:
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Animal science journal = Nihon chikusan Gakkaiho [Anim Sci J] 2018 Jan; Vol. 89 (1), pp. 31-41. Date of Electronic Publication: 2017 Aug 18.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Richmond, Vic. : Wiley
Original Publication: Tokyo, Japan : Japanese Society of Zootechnical Science [1999-
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MeSH Terms:
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Cloning, Organism*
Gas Chromatography-Mass Spectrometry*
Amino Acid Transport Systems/*genetics
Amino Acid Transport Systems/*metabolism
Culture Media/*chemistry
Embryonic Development/*physiology
Goats/*genetics
Goats/*metabolism
Metabolomics/*methods
Amino Acid Transport System ASC ; Amino Acids/analysis ; Animals ; Blastocyst ; Embryo Culture Techniques/methods ; Fusion Regulatory Protein 1, Heavy Chain ; Gene Expression ; Glucose/analysis ; Minor Histocompatibility Antigens ; Phosphates/analysis ; Polymerase Chain Reaction ; Sphingosine/analogs & derivatives ; Sphingosine/analysis
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Contributed Indexing:
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Keywords: GC-MS; cloned embryo; culture media; goat; metabolomics
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Substance Nomenclature:
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0 (Amino Acid Transport System ASC)
0 (Amino Acid Transport Systems)
0 (Amino Acids)
0 (Culture Media)
0 (Fusion Regulatory Protein 1, Heavy Chain)
0 (Minor Histocompatibility Antigens)
0 (Phosphates)
0 (SLC1A5 protein, human)
GIN46U9Q2Q (phytosphingosine)
IY9XDZ35W2 (Glucose)
NGZ37HRE42 (Sphingosine)
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Entry Date(s):
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Date Created: 20170824 Date Completed: 20180222 Latest Revision: 20180222
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Update Code:
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20240105
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DOI:
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10.1111/asj.12885
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PMID:
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28833899
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Pre-implantation embryo metabolism demonstrates distinctive characteristics associated with the development potential of embryos. We aim to determine if metabolic differences correlate with embryo morphology. In this study, gas chromatography - mass spectroscopy (GC-MS)-based metabolomics was used to assess the culture media of goat cloned embryos collected from high-quality (HQ) and low-quality (LQ) groups based on morphology. Expression levels of amino acid transport genes were further examined by quantitative real-time PCR. Results showed that the HQ group presented higher percentages of blastocysts compared with the LQ counterparts (P < 0.05). Metabolic differences were also present between HQ and LQ groups. The culture media of the HQ group showed lower levels of valin, lysine, glutamine, mannose and acetol, and higher levels of glucose, phytosphingosine and phosphate than those of the LQ group. Additionally, expression levels of amino acid transport genes SLC1A5 and SLC3A2 were significantly lower in the HQ group than the LQ group (P < 0.05, respectively). To our knowledge, this is the first report which uses GC-MS to detect metabolic differences in goat cloned embryo culture media. The biochemical profiles may help to select the most in vitro viable embryos.
(© 2017 Japanese Society of Animal Science.)
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