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Tytuł:
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A Novel, Low-Cost Anesthesia and Injection System for Zebrafish Researchers.
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Autorzy:
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Oskay Y; 1 Mechanical Engineering Department, İ.D. Bilkent University , Ankara, Turkey .
Çetin B; 1 Mechanical Engineering Department, İ.D. Bilkent University , Ankara, Turkey .
Şerifoğlu N; 2 Interdisciplinary Graduate Program in Neuroscience, Aysel Sabuncu Brain Research Center, Bilkent University , Ankara, Turkey .; 3 UNAM-National Nanotechnology Research Center and Institute of Materials Science and Nanotechnology, Bilkent University , Ankara, Turkey .; 4 Department of Molecular Biology and Genetics Department Zebrafish Facility, Bilkent University , Ankara, Turkey .
Arslan-Ergül A; 2 Interdisciplinary Graduate Program in Neuroscience, Aysel Sabuncu Brain Research Center, Bilkent University , Ankara, Turkey .; 3 UNAM-National Nanotechnology Research Center and Institute of Materials Science and Nanotechnology, Bilkent University , Ankara, Turkey .; 4 Department of Molecular Biology and Genetics Department Zebrafish Facility, Bilkent University , Ankara, Turkey .; 5 Hacettepe University , Transgenic Animal Technology A.R.C, Ankara, Turkey .
Adams MM; 2 Interdisciplinary Graduate Program in Neuroscience, Aysel Sabuncu Brain Research Center, Bilkent University , Ankara, Turkey .; 3 UNAM-National Nanotechnology Research Center and Institute of Materials Science and Nanotechnology, Bilkent University , Ankara, Turkey .; 4 Department of Molecular Biology and Genetics Department Zebrafish Facility, Bilkent University , Ankara, Turkey .; 6 Department of Psychology, Bilkent University , Ankara, Turkey .
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Źródło:
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Zebrafish [Zebrafish] 2018 Apr; Vol. 15 (2), pp. 85-95. Date of Electronic Publication: 2017 Dec 20.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c2004-
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MeSH Terms:
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Anesthesia/*veterinary
Injections, Intraperitoneal/*veterinary
Anesthesia/economics ; Anesthesia/methods ; Animals ; Injections, Intraperitoneal/economics ; Injections, Intraperitoneal/instrumentation ; Injections, Intraperitoneal/methods ; Zebrafish
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Contributed Indexing:
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Keywords: Peltier coolers; gradual cold water anesthesia; injection bed system; intraperitoneal injection
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Entry Date(s):
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Date Created: 20171221 Date Completed: 20180703 Latest Revision: 20180703
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Update Code:
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20240104
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DOI:
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10.1089/zeb.2017.1513
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PMID:
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29261032
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In this study, we designed and developed a novel low-cost system for anesthetizing and injecting adult zebrafish. The system utilizes a gradual cooling method for the anesthesia and maintains the fish in a stable anesthetic plane, as well as stabilizes the animal so that intraperitoneal injections can be consistently performed. It is a system that any laboratory with access to a workshop can build for their group. Moreover, it is a safe system for researchers, as well as a reliable one for repeated experiments since multiple fish can be injected quickly and there is little physical contact necessary between the investigator and the animal. This will likely reduce any unnecessary stress in the fish, as compared with manual methods of injection. Finally, the system is adaptable so that as the investigators' procedural needs change due to different research questions, that is, gradual rewarming or something of that nature, it could be modified.