-
Tytuł:
-
Induction of apoptosis in nasal polyp-derived fibroblasts by bleomycin A5 in vitro.
-
Autorzy:
-
Wu F; Department of Otorhinolaryngology‑Head and Neck Surgery, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China.
Tian P; Department of Otorhinolaryngology‑Head and Neck Surgery, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China.
Ma Y; Department of Otorhinolaryngology‑Head and Neck Surgery, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China.
Wang J; Department of Otorhinolaryngology‑Head and Neck Surgery, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China.
Ou H; Department of Otorhinolaryngology‑Head and Neck Surgery, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China.
Zou H; Department of Otorhinolaryngology‑Head and Neck Surgery, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China.
-
Źródło:
-
Molecular medicine reports [Mol Med Rep] 2018 Apr; Vol. 17 (4), pp. 5384-5389. Date of Electronic Publication: 2018 Feb 01.
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Original Publication: Athens, Greece : D. A. Spandidos
-
MeSH Terms:
-
Apoptosis/*drug effects
Bleomycin/*analogs & derivatives
Fibroblasts/*drug effects
Fibroblasts/*metabolism
Nasal Polyps/*metabolism
Nasal Polyps/*pathology
Adult ; Biomarkers ; Bleomycin/pharmacology ; Caspases/metabolism ; Cell Survival/drug effects ; Cells, Cultured ; Dose-Response Relationship, Drug ; Female ; Gene Expression Profiling ; Humans ; Male ; Nasal Polyps/genetics ; Poly(ADP-ribose) Polymerases/metabolism ; Proto-Oncogene Proteins c-bcl-2/metabolism
-
Contributed Indexing:
-
Keywords: nasal polyps; fibroblasts; bleomycin A5; apoptosis
-
Substance Nomenclature:
-
0 (Biomarkers)
0 (Proto-Oncogene Proteins c-bcl-2)
11056-06-7 (Bleomycin)
5DY91Y7601 (bleomycetin)
EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
EC 3.4.22.- (Caspases)
-
Entry Date(s):
-
Date Created: 20180203 Date Completed: 20180827 Latest Revision: 20211105
-
Update Code:
-
20240104
-
DOI:
-
10.3892/mmr.2018.8540
-
PMID:
-
29393498
-
The present study aimed to evaluate the pro-apoptotic effects of bleomycin A5 on nasal polyp‑derived fibroblasts (NPDFs) and the underlying molecular mechanisms. Nasal polyp tissue was acquired from 10 patients during surgery and NPDFs were isolated from surgical tissues. Fibroblasts were identified using immunohistochemistry. Bleomycin A5 was used to treat NPDFs along a concentration gradient. Cell viability was evaluated using a Cell Counting Kit‑8 assay. A flow cytometric Annexin V‑fluorescein isothiocyanate/propidium iodide assay was used to determine the percentage of apoptotic NPDFs. The mRNA expression levels of apoptotic genes were determined by reverse transcription‑quantitative polymerase chain reaction and levels of proteins associated with apoptosis were determined by western blotting. The results indicated that bleomycin A5 was able to induce apoptosis in NPDFs in a dose‑dependent manner. NPDFs treated with bleomycin A5 were identified to contain significantly high amounts of the active forms of caspase‑3 and showed considerable cleavage of poly(ADP‑ribose) polymerase. The mRNA and protein expression levels of the pro‑apoptotic molecule Bcl‑2‑associated X protein were significantly higher in treated NPDFs than in untreated NPDFs. In contrast, the mRNA and protein expression of the anti‑apoptotic molecule B‑cell lymphoma 2 (Bcl‑2) was significantly lower in treated NPDFs. These results indicated that bleomycin A5 could induce apoptosis in primary NPDFs through activation of the Bcl‑2 family and caspase cascades in a time-, and concentration-dependent manner.
Zaloguj się, aby uzyskać dostęp do pełnego tekstu.