Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Przeglądasz jako GOŚĆ
Tytuł pozycji:

Overcoming the polycation dilemma - Explorative studies to characterise the efficiency and biocompatibility of newly designed lipofection reagents.

Tytuł :
Overcoming the polycation dilemma - Explorative studies to characterise the efficiency and biocompatibility of newly designed lipofection reagents.
Autorzy :
Giselbrecht J; Martin Luther University Halle-Wittenberg, Institute of Pharmacy, Wolfgang-Langenbeck-Strasse 4, 06120 Halle (Saale), Germany.
Janich C; Martin Luther University Halle-Wittenberg, Institute of Pharmacy, Wolfgang-Langenbeck-Strasse 4, 06120 Halle (Saale), Germany.
Pinnapireddy SR; Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Robert-Koch-Strasse 4, 35037 Marburg, Germany.
Hause G; Martin Luther University Halle-Wittenberg, Biocenter, Weinbergweg 22, 06120 Halle, Germany.
Bakowsky U; Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Robert-Koch-Strasse 4, 35037 Marburg, Germany.
Wölk C; Martin Luther University Halle-Wittenberg, Institute of Pharmacy, Wolfgang-Langenbeck-Strasse 4, 06120 Halle (Saale), Germany. Electronic address: .
Langner A; Martin Luther University Halle-Wittenberg, Institute of Pharmacy, Wolfgang-Langenbeck-Strasse 4, 06120 Halle (Saale), Germany.
Pokaż więcej
Źródło :
International journal of pharmaceutics [Int J Pharm] 2018 Apr 25; Vol. 541 (1-2), pp. 81-92. Date of Electronic Publication: 2018 Feb 17.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
MeSH Terms :
DNA/*chemistry
Lipids/*chemistry
Phosphatidylethanolamines/*chemistry
Transfection/*methods
Cations/chemistry ; Chemistry, Pharmaceutical ; Colloids/chemistry ; Genetic Therapy/methods ; Liposomes ; Polyamines/chemistry ; Polyelectrolytes
Contributed Indexing :
Keywords: Cationic lipids; Gene therapy; Haemocompatibility; Lipofection; Lipoplex; Serum stability
Substance Nomenclature :
0 (Cations)
0 (Colloids)
0 (Lipids)
0 (Liposomes)
0 (Phosphatidylethanolamines)
0 (Polyamines)
0 (Polyelectrolytes)
0 (polycations)
2462-63-7 (dioleoyl phosphatidylethanolamine)
9007-49-2 (DNA)
Entry Date(s) :
Date Created: 20180221 Date Completed: 20180907 Latest Revision: 20201209
Update Code :
20210301
DOI :
10.1016/j.ijpharm.2018.02.029
PMID :
29462683
Czasopismo naukowe
In this explorative study of the novel cationic lipid OO4 in two different formulations the complex formation with DNA, the biopharmaceutical stability of the lipid/DNA complexes in physiological media, and the transfection efficiency were analysed. We investigated liposomes composed of two binary mixtures of OO4 with either DOPE or DPPE as co-lipids in the molar ratio of 1:3. These formulations were compared with regard to their ability to bind the DNA using gel retardation electrophoresis, ethidium bromide exclusion and zeta potential measurements. Colloidal stability of the lipoplexes in foetal bovine serum (FBS) and the protective effect against degradation by endonucleases were studied. Furthermore, the influence of different salt concentrations on the complex formation with DNA was examined. The DOPE mixture was markedly superior compared to the DPPE mixture. Finally, haemocompatibility studies and gene silencing experiments were performed on OO4:DOPE 1:3 (n:n). The experiments demonstrate that the lipoplex formulation OO4:DOPE 1:3 (n:n) at N/P 4 is a promising candidate for systemic application because of the high colloidal stability in serum without PEGylated lipids, high transfection efficiency, superior resistance against nucleases, reproducible complexation independent of ionic effects, and haemocompatibility.
(Copyright © 2018 Elsevier B.V. All rights reserved.)

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies