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Tytuł:
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Intralesional bleomycin A5 injection for the treatment of nasal polyps through inducing apoptosis.
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Autorzy:
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Tian P; a Department of Otorhinolaryngology-Head and Neck Surgery , Sun Yat-sen Memorial Hospital, Sun Yat-sen University , Guangzhou , Guangdong , People's Republic of China.
Wu F; a Department of Otorhinolaryngology-Head and Neck Surgery , Sun Yat-sen Memorial Hospital, Sun Yat-sen University , Guangzhou , Guangdong , People's Republic of China.
Wang J; a Department of Otorhinolaryngology-Head and Neck Surgery , Sun Yat-sen Memorial Hospital, Sun Yat-sen University , Guangzhou , Guangdong , People's Republic of China.
Ou H; a Department of Otorhinolaryngology-Head and Neck Surgery , Sun Yat-sen Memorial Hospital, Sun Yat-sen University , Guangzhou , Guangdong , People's Republic of China.
Liu X; a Department of Otorhinolaryngology-Head and Neck Surgery , Sun Yat-sen Memorial Hospital, Sun Yat-sen University , Guangzhou , Guangdong , People's Republic of China.
Chen Q; a Department of Otorhinolaryngology-Head and Neck Surgery , Sun Yat-sen Memorial Hospital, Sun Yat-sen University , Guangzhou , Guangdong , People's Republic of China.
Dang H; a Department of Otorhinolaryngology-Head and Neck Surgery , Sun Yat-sen Memorial Hospital, Sun Yat-sen University , Guangzhou , Guangdong , People's Republic of China.
Zheng Y; a Department of Otorhinolaryngology-Head and Neck Surgery , Sun Yat-sen Memorial Hospital, Sun Yat-sen University , Guangzhou , Guangdong , People's Republic of China.
Zhang X; b Department of Otorhinolaryngology-Head and Neck Surgery , Fu Xing Hospital Affiliated to Capital University of Medical Sciences , Beijing , People's Republic of China.
Zou H; a Department of Otorhinolaryngology-Head and Neck Surgery , Sun Yat-sen Memorial Hospital, Sun Yat-sen University , Guangzhou , Guangdong , People's Republic of China.
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Źródło:
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Acta oto-laryngologica [Acta Otolaryngol] 2018 May; Vol. 138 (5), pp. 475-482. Date of Electronic Publication: 2018 Feb 28.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: 2015- : Abingdon, Oxford : Taylor & Francis
Original Publication: Stockholm : Scandinavian University Press
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MeSH Terms:
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Apoptosis/*drug effects
Bleomycin/*analogs & derivatives
Nasal Polyps/*drug therapy
Adult ; Bleomycin/pharmacology ; Bleomycin/therapeutic use ; Caspase 8/metabolism ; Female ; Follow-Up Studies ; Humans ; In Situ Nick-End Labeling ; Injections, Intralesional ; Male ; Middle Aged ; Nasal Polyps/metabolism ; Poly(ADP-ribose) Polymerases/metabolism ; Retrospective Studies
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Contributed Indexing:
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Keywords: Nasal polyp; apoptosis; bleomycin A5
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Substance Nomenclature:
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11056-06-7 (Bleomycin)
5DY91Y7601 (bleomycetin)
EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
EC 3.4.22.- (CASP8 protein, human)
EC 3.4.22.- (Caspase 8)
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Entry Date(s):
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Date Created: 20180302 Date Completed: 20180926 Latest Revision: 20180926
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Update Code:
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20240104
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DOI:
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10.1080/00016489.2017.1380312
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PMID:
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29490557
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Conclusion: An intralesional bleomycin A5 (BLE) injection might be used as an alternative therapy for eosinophilic-type nasal polyps (NPs). BLE-induced apoptosis might play an important role in shrinkage of NPs.
Objectives: The aim of this study is to determine the effect and explore the possible role of apoptosis in shrinkage of NPs.
Methods: Twenty-nine patients with eosinophilic-type NPs experienced repeated local injection of BLE. The recurrence rate of this group was obtained. The mechanism of BLE treatment was investigated through an in vitro experiment. Nasal polyp tissues were treated with BLE. The apoptotic activity was detected by the presence of DNA smear and test of terminal deoxynucleotidyl transferase dUTP nick end labeling. The caspase-8 and PARP were examined through immunohistochemistry and Western blotting.
Results: After several local injections of BLE, the nasal polyp tissues decreased and then disappeared. During follow-up of 3 years the recurrence rate of this group was significantly lower than another one treated with operation plus medicine treatment. Apoptosis in BLE-treated tissue was prominently detected in the infiltrating inflammatory cells. The expression of PARP and casp-8 were increased in BLE-treated nasal polyp tissue compared with PBS-treated tissue.
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