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Tytuł:
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Next generation sequencing for preimplantation genetic screening improves pregnancy outcomes compared with array comparative genomic hybridization in single thawed euploid embryo transfer cycles.
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Autorzy:
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Friedenthal J; New York University Langone Fertility Center, New York, New York. Electronic address: .
Maxwell SM; New York University Langone Fertility Center, New York, New York.
Munné S; Cooper Genomics, Livingston, New Jersey.
Kramer Y; New York University Langone Fertility Center, New York, New York.
McCulloh DH; New York University Langone Fertility Center, New York, New York.
McCaffrey C; New York University Langone Fertility Center, New York, New York.
Grifo JA; New York University Langone Fertility Center, New York, New York.
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Źródło:
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Fertility and sterility [Fertil Steril] 2018 Apr; Vol. 109 (4), pp. 627-632. Date of Electronic Publication: 2018 Mar 28.
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Typ publikacji:
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Comparative Study; Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: New York. NY : Elsevier for the American Society for Reproductive Medicine
Original Publication: New York, Hoeber.
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MeSH Terms:
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Comparative Genomic Hybridization*
Cryopreservation*
Fertilization in Vitro*/adverse effects
High-Throughput Nucleotide Sequencing*
Single Embryo Transfer*/adverse effects
Blastocyst/*pathology
Genetic Testing/*methods
Infertility/*therapy
Preimplantation Diagnosis/*methods
Abortion, Spontaneous/etiology ; Abortion, Spontaneous/genetics ; Adult ; Embryo Implantation ; Female ; Fertility ; Humans ; Infertility/diagnosis ; Infertility/genetics ; Infertility/physiopathology ; Live Birth ; Mosaicism ; Predictive Value of Tests ; Pregnancy ; Pregnancy Outcome ; Reproducibility of Results ; Retrospective Studies ; Treatment Outcome
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Contributed Indexing:
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Keywords: Next generation sequencing; array comparative genomic hybridization; mosaicism; preimplantation genetic screening
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Entry Date(s):
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Date Created: 20180402 Date Completed: 20181211 Latest Revision: 20181211
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Update Code:
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20240105
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DOI:
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10.1016/j.fertnstert.2017.12.017
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PMID:
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29605407
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Objective: To evaluate whether the use of next generation sequencing (NGS) for preimplantation genetic screening (PGS) in single thawed euploid embryo transfer (STEET) cycles improves pregnancy outcomes compared with array comparative genomic hybridization (aCGH).
Design: Retrospective cohort study.
Setting: Single university-based fertility center.
Patient(s): A total of 916 STEET cycles from January 2014 to December 2016 were identified. Cases included 548 STEET cycles using NGS for PGS and controls included 368 STEET cycles using aCGH for PGS.
Intervention(s): Patients having a STEET after undergoing IVF and PGS with either NGS or aCGH.
Main Outcome Measure(s): Primary outcomes were implantation rate, ongoing pregnancy/live birth rate (OP/LBR), biochemical pregnancy rate (PR), and spontaneous abortion (SAB) rate.
Result(s): The implantation rate was significantly higher in the NGS group compared with the aCGH group (71.6% vs. 64.6%). The OP/LBR was also significantly higher in the NGS group (62% vs. 54.4%), and there were significantly more biochemical pregnancies in the aCGH group compared with the NGS group (15.1% vs. 8.7%). After adjustment for confounding variables with a multiple logistic regression analysis, OP/LBR remained significantly higher in the NGS group. The SAB rate was not significantly different in the NGS group compared with the aCGH group (12.4% vs. 12.7%).
Conclusion(s): Preimplantation genetic screening using NGS significantly improves pregnancy outcomes versus PGS using aCGH in STEET cycles. Next-generation sequencing has the ability to identify and screen for embryos with reduced viability such as mosaic embryos and those with partial aneuploidies or triploidy. Pregnancy outcomes with NGS may be improved due to the exclusion of these abnormal embryos.
(Copyright © 2017 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.)
Comment in: Fertil Steril. 2018 Apr;109(4):606-607. (PMID: 29653707)
