-
Tytuł:
-
Stress-related salivary proteins affect the production of volatile sulfur compounds by oral bacteria.
-
Autorzy:
-
de Lima PO; Department of Physiological Sciences, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, SP, Brazil.
Nani BD; Department of Physiological Sciences, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, SP, Brazil.
Almeida B; Department of Physiological Sciences, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, SP, Brazil.
Marcondes FK; Department of Physiological Sciences, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, SP, Brazil.
Groppo FC; Department of Physiological Sciences, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, SP, Brazil.
de Moraes ABA; Department of Physiological Sciences, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, SP, Brazil.
Franz-Montan M; Department of Physiological Sciences, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, SP, Brazil.
Cogo-Müller K; Department of Physiological Sciences, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, SP, Brazil.; Faculty of Pharmaceutical Sciences, University of Campinas - UNICAMP, Campinas, SP, Brazil.
-
Źródło:
-
Oral diseases [Oral Dis] 2018 Oct; Vol. 24 (7), pp. 1358-1366. Date of Electronic Publication: 2018 May 29.
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Publication: 2001- : Copenhagen, Denmark : Munksgaard
Original Publication: Houndmills, Basingstoke, Hampshire, UK : Stockton Press, c1995-
-
MeSH Terms:
-
Fusobacterium nucleatum/*metabolism
Gram-Positive Bacteria/*metabolism
Hydrogen Sulfide/*metabolism
Mouth/*microbiology
Salivary Proteins and Peptides/*metabolism
Sulfhydryl Compounds/*metabolism
Estradiol/metabolism ; Humans ; Mucins/metabolism ; Progesterone/metabolism ; alpha-Amylases/metabolism ; beta-Defensins/metabolism
-
Grant Information:
-
2011/50419-2 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2013/26691-0 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2014/11481-2 Fundação de Amparo à Pesquisa do Estado de São Paulo
-
Contributed Indexing:
-
Keywords: Fusobacterium nucleatum; Solobacterium moorei; alpha-amylase; hydrogen sulfide; mucin
-
Substance Nomenclature:
-
0 (Mucins)
0 (Salivary Proteins and Peptides)
0 (Sulfhydryl Compounds)
0 (beta-Defensins)
2X8406WW9I (methylmercaptan)
4G7DS2Q64Y (Progesterone)
4TI98Z838E (Estradiol)
EC 3.2.1.1 (alpha-Amylases)
YY9FVM7NSN (Hydrogen Sulfide)
-
Entry Date(s):
-
Date Created: 20180516 Date Completed: 20181217 Latest Revision: 20181217
-
Update Code:
-
20240104
-
DOI:
-
10.1111/odi.12890
-
PMID:
-
29761905
-
Objective: To determine whether stress-related substances and sex hormones influence the growth and in vitro production of volatile sulfur compounds (VSCs) by Solobacterium moorei and Fusobacterium nucleatum.
Materials and Methods: Bacteria growth and VSCs production were evaluated in the presence of alpha-amylase, beta-defensin-2, mucin, estradiol, and progesterone. Growth was evaluated by colony counting, and the production of the VSCs hydrogen sulfide (H 2 S) and methyl mercaptan (CH 3 SH) was measured using the Oral Chroma™ instrument.
Results: Mucin induced the production of H 2 S by both bacteria, but had a slight inhibitory effect on CH 3 SH production by F. nucleatum. It also increased the viability of F. nucleatum. Alpha-amylase increased H 2 S production by S. moorei and CH 3 SH production by F. nucleatum, but had no effect on H 2 S production by F. nucleatum. No substance altered the viability of S. moorei. No effects of beta-defensin-2, estradiol, or progesterone were observed.
Conclusion: The salivary stress-related proteins mucin and alpha-amylase altered VSCs production by F. nucleatum and S. moorei, favoring H 2 S production. These findings are a step toward understanding the relation between stress and increased amounts of H 2 S.
(© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.)
