-
Tytuł:
-
Extracts of compound Muniziqi granule suppressed uterus contraction and ameliorated oxytocin-induced primary dysmenorrhea.
-
Autorzy:
-
Wei Y; Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine; The MOE Key Laboratory for Standardization of Chinese Medicines; Shanghai Key Laboratory of Compound Chinese Medicines; Shanghai R&D Centre for Standardization of Chinese Medicines, 1200 Cailun Rood, Shanghai 201203, China.
Ma T; Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine; The MOE Key Laboratory for Standardization of Chinese Medicines; Shanghai Key Laboratory of Compound Chinese Medicines; Shanghai R&D Centre for Standardization of Chinese Medicines, 1200 Cailun Rood, Shanghai 201203, China.
Wang H; Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine; The MOE Key Laboratory for Standardization of Chinese Medicines; Shanghai Key Laboratory of Compound Chinese Medicines; Shanghai R&D Centre for Standardization of Chinese Medicines, 1200 Cailun Rood, Shanghai 201203, China.
Xing J; The Xinjiang Institute of Materia Medica, Urumqi, Xinjiang 830002, China.
Wang Y; Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine; The MOE Key Laboratory for Standardization of Chinese Medicines; Shanghai Key Laboratory of Compound Chinese Medicines; Shanghai R&D Centre for Standardization of Chinese Medicines, 1200 Cailun Rood, Shanghai 201203, China.
Gu Z; The Xinjiang Institute of Materia Medica, Urumqi, Xinjiang 830002, China.
Mu D; Xinjiang Uighur Pharmaceutical Limited Company, Urumqi, Xinjiang 830001, China.
Yin Q; Xinjiang Uighur Pharmaceutical Limited Company, Urumqi, Xinjiang 830001, China.
Cheng X; Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine; The MOE Key Laboratory for Standardization of Chinese Medicines; Shanghai Key Laboratory of Compound Chinese Medicines; Shanghai R&D Centre for Standardization of Chinese Medicines, 1200 Cailun Rood, Shanghai 201203, China. Electronic address: .
Wang C; Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine; The MOE Key Laboratory for Standardization of Chinese Medicines; Shanghai Key Laboratory of Compound Chinese Medicines; Shanghai R&D Centre for Standardization of Chinese Medicines, 1200 Cailun Rood, Shanghai 201203, China; The Xinjiang Institute of Materia Medica, Urumqi, Xinjiang 830002, China. Electronic address: .
-
Źródło:
-
Journal of ethnopharmacology [J Ethnopharmacol] 2018 Sep 15; Vol. 223, pp. 33-40. Date of Electronic Publication: 2018 May 19.
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Publication: Limerick : Elsevier Sequoia
Original Publication: Lausanne, Elsevier Sequoia.
-
MeSH Terms:
-
Analgesics/*pharmacology
Analgesics/*therapeutic use
Dysmenorrhea/*drug therapy
Plant Extracts/*pharmacology
Plant Extracts/*therapeutic use
Uterine Contraction/*drug effects
Animals ; Dysmenorrhea/chemically induced ; Dysmenorrhea/physiopathology ; Female ; Mice ; Nigella ; Oxytocin ; Peganum ; Phytotherapy ; Seeds ; Uterus/drug effects ; Uterus/physiology
-
Contributed Indexing:
-
Keywords: Analgesic activity; Compound Muniziqi granule; Nigella glandulifera; Peganum harmala; Primary dysmenorrhea; Uterine contraction
-
Substance Nomenclature:
-
0 (Analgesics)
0 (Plant Extracts)
50-56-6 (Oxytocin)
-
Entry Date(s):
-
Date Created: 20180522 Date Completed: 20181018 Latest Revision: 20181018
-
Update Code:
-
20240104
-
DOI:
-
10.1016/j.jep.2018.05.024
-
PMID:
-
29783015
-
Ethnopharmacological Relevance: Compound Muniziqi granule (CMG) is usually used as a traditional Uighur medicine to treat acne, chloasma, skin inflammation, primary dysmenorrhea (PDM), and menopausal syndrome. However, there are no sufficient data to support the clinic uses of CMG in PDM.
Aim of the Study: This work aims to examine the effect of CMG as a treatment for PDM and reveal its possible therapeutic mechanism.
Materials and Methods: In vivo and in vitro mouse PDM models were utilized in this study. The mouse uterine contraction was induced by oxytocin after progynova or estradiol benzoate pretreatment. CMG, alkaloid extracts from seeds of Peganum harmala (AEP), and 10% and 95% ethanol extracts from seeds of Nigella glandulifera (EEN10 and EEN95) were given to mice in three doses by gavage. The writhing times within 30 min after oxytocin treatment were recorded to evaluate the analgesic effect, and the glutathione peroxidase (GSH-Px), malondialdehyde (MDA), 6-keto-prostaglandin F 1α (6-k-PGF 1α ), prostaglandin F 2α (PGF 2α ), thromboxane B2 (TXB 2 ), and nitric oxide (NO) levels in uterine tissues and PGF 2α and MDA in serum were determined. The effects (contractile curve) of CMG, AEP, EEN10, and EEN95 on uterus contraction induced by oxytocin in isolated mouse uterus were recorded.
Results: In contrast to the control group, CMG, AEP, N10, and N95 could display analgesic activities dose dependently by reducing the writhing response of the PDM model mice. CMG, AEP, EEN10, and EEN95 could also remarkably decrease the level of PGF 2α , 6-k-PGF 1α , TXB 2 , NO and MDA in uterine tissues and PGF 2α and MDA in serum, whereas the activity of GSH-Px in uterine tissues was increased. Furthermore, CMG, AEP, EEN10, and EEN95 could significantly inhibit the frequency and amplitude of isolated uterus induced by oxytocin in a concentration-dependent manner.
Conclusions: CMG exhibited a significant protective effect on experimental PDM. The mechanisms are probably associated with abating lipid peroxidation and over-inflammatory reaction, and alleviating the contraction of isolated mouse uterus. The seeds of P. harmala and N. glandulifera in the CMG may play an important role in exerting protective effects on PDM. This study provides pre-clinic proof to the use of CMG in clinical practice of PDM.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
