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Title:
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Cantilever Functionalization Using Peroxidase Extract of Low Cost for Glyphosate Detection.
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Authors:
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Muenchen DK; Department of Food Engineering, URI - Erechim, Av. Sete de Setembro, 1621, Erechim, Rio Grande do Sul, 99709-910, Brazil.
Martinazzo J; Department of Food Engineering, URI - Erechim, Av. Sete de Setembro, 1621, Erechim, Rio Grande do Sul, 99709-910, Brazil.
Brezolin AN; Department of Food Engineering, URI - Erechim, Av. Sete de Setembro, 1621, Erechim, Rio Grande do Sul, 99709-910, Brazil.
de Cezaro AM; Department of Food Engineering, URI - Erechim, Av. Sete de Setembro, 1621, Erechim, Rio Grande do Sul, 99709-910, Brazil.
Rigo AA; Department of Food Engineering, URI - Erechim, Av. Sete de Setembro, 1621, Erechim, Rio Grande do Sul, 99709-910, Brazil.
Mezarroba MN; Department of Food Engineering, URI - Erechim, Av. Sete de Setembro, 1621, Erechim, Rio Grande do Sul, 99709-910, Brazil.
Manzoli A; Department of Food Engineering, URI - Erechim, Av. Sete de Setembro, 1621, Erechim, Rio Grande do Sul, 99709-910, Brazil.
de Lima Leite F; Department of Physics, Chemistry and Mathematics, Nanoneurobiophysics Research Group, Federal University of São Carlos (UFSCar), P.O. Box 3031, Sorocaba, São Paulo, 18052-780, Brazil.
Steffens J; Department of Food Engineering, URI - Erechim, Av. Sete de Setembro, 1621, Erechim, Rio Grande do Sul, 99709-910, Brazil.
Steffens C; Department of Food Engineering, URI - Erechim, Av. Sete de Setembro, 1621, Erechim, Rio Grande do Sul, 99709-910, Brazil. .
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Source:
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Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2018 Dec; Vol. 186 (4), pp. 1061-1073. Date of Electronic Publication: 2018 Jun 04.
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Publication Type:
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Journal Article
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Language:
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English
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Imprint Name(s):
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Original Publication: Clifton, N.J. : Humana Press, c1981-
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MeSH Terms:
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Biosensing Techniques/*methods
Cucurbita/*enzymology
Glycine/*analogs & derivatives
Peroxidase/*chemistry
Plant Proteins/*chemistry
Glycine/analysis ; Glyphosate
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Contributed Indexing:
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Keywords: Atomic force microscopy (AFM); Enzymatic activity; Functionalization; Nanobiosensor; Self-assembled monolayers (SAM); Zucchini
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Substance Nomenclature:
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0 (Plant Proteins)
EC 1.11.1.7 (Peroxidase)
TE7660XO1C (Glycine)
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Entry Date(s):
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Date Created: 20180605 Date Completed: 20181126 Latest Revision: 20231213
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Update Code:
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20240105
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DOI:
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10.1007/s12010-018-2799-y
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PMID:
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29862444
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A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10 mg L -1 ) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028 mg L -1 .
