-
Tytuł:
-
The transcription factor Tfap2e/AP-2ε plays a pivotal role in maintaining the identity of basal vomeronasal sensory neurons.
-
Autorzy:
-
Lin JM; Department of Biological Sciences, University at Albany, Albany, NY 12222, USA.
Taroc EZM; Department of Biological Sciences, University at Albany, Albany, NY 12222, USA.
Frias JA; Department of Biological Sciences, University at Albany, Albany, NY 12222, USA.
Prasad A; Department of Biological Sciences, University at Albany, Albany, NY 12222, USA.
Catizone AN; Department of Biological Sciences, University at Albany, Albany, NY 12222, USA.
Sammons MA; Department of Biological Sciences, University at Albany, Albany, NY 12222, USA.
Forni PE; Department of Biological Sciences, University at Albany, Albany, NY 12222, USA. Electronic address: .
-
Źródło:
-
Developmental biology [Dev Biol] 2018 Sep 01; Vol. 441 (1), pp. 67-82. Date of Electronic Publication: 2018 Jun 19.
-
Typ publikacji:
-
Journal Article; Research Support, Non-U.S. Gov't
-
Język:
-
English
-
Imprint Name(s):
-
Publication: San Diego, CA : Elsevier
Original Publication: New York.
-
MeSH Terms:
-
GABAergic Neurons/*metabolism
Gene Expression Regulation, Developmental/*physiology
Nasal Mucosa/*embryology
Sensory Receptor Cells/*metabolism
Transcription Factor AP-2/*biosynthesis
Vomeronasal Organ/*embryology
Animals ; Cell Differentiation/physiology ; Mice ; Mice, Transgenic ; Mutation ; Nasal Mucosa/cytology ; Sensory Receptor Cells/cytology ; Transcription Factor AP-2/genetics ; Vomeronasal Organ/cytology
-
Substance Nomenclature:
-
0 (Transcription Factor AP-2)
-
Entry Date(s):
-
Date Created: 20180622 Date Completed: 20180807 Latest Revision: 20180807
-
Update Code:
-
20240104
-
DOI:
-
10.1016/j.ydbio.2018.06.007
-
PMID:
-
29928868
-
The identity of individual neuronal cell types is defined and maintained by the expression of specific combinations of transcriptional regulators that control cell type-specific genetic programs. The epithelium of the vomeronasal organ of mice contains two major types of vomeronasal sensory neurons (VSNs): 1) the apical VSNs which express vomeronasal 1 receptors (V1r) and the G-protein subunit Gαi2 and; 2) the basal VSNs which express vomeronasal 2 receptors (V2r) and the G-protein subunit Gαo. Both cell types originate from a common pool of progenitors and eventually acquire apical or basal identity through largely unknown mechanisms. The transcription factor AP-2ε, encoded by the Tfap2e gene, plays a role in controlling the development of GABAergic interneurons in the main and accessory olfactory bulb (AOB), moreover AP-2ε has been previously described to be expressed in the basal VSNs. Here we show that AP-2ε is expressed in post-mitotic VSNs after they commit to the basal differentiation program. Loss of AP-2ε function resulted in reduced number of basal VSNs and in an increased number of neurons expressing markers of the apical lineage. Our work suggests that AP-2ε, which is expressed in late phases of differentiation, is not needed to initiate the apical-basal differentiation dichotomy but for maintaining the basal VSNs' identity. In AP-2ε mutants we observed a large number of cells that entered the basal program can express apical genes, our data suggest that differentiated VSNs of mice retain a notable level of plasticity.
(Copyright © 2018 Elsevier Inc. All rights reserved.)