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Tytuł:
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Delivery efficiencies of constituents of combustion-derived aerosols across the air-liquid interface during in vitro exposures.
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Autorzy:
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Steiner S; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland. Electronic address: .
Diana P; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland. Electronic address: .
Dossin E; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland. Electronic address: .
Guy P; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland. Electronic address: .
Vuillaume G; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland. Electronic address: .
Kondylis A; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland. Electronic address: .
Majeed S; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland. Electronic address: .
Frentzel S; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland. Electronic address: .
Hoeng J; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland. Electronic address: .
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Źródło:
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Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2018 Oct; Vol. 52, pp. 384-398. Date of Electronic Publication: 2018 Jul 09.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Oxford ; New York : Pergamon Press, c1987-
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MeSH Terms:
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Cell Culture Techniques*
Tobacco Products*
Epithelial Cells/*drug effects
Smoke/*adverse effects
Toxicity Tests/*methods
Aerosols ; Smoke/analysis
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Contributed Indexing:
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Keywords: Aerosol; Aerosol dose; Cigarette smoke exposure; Combustion derived aerosol; In vitro exposure
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Substance Nomenclature:
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0 (Aerosols)
0 (Smoke)
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Entry Date(s):
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Date Created: 20180714 Date Completed: 20181126 Latest Revision: 20181126
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Update Code:
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20240105
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DOI:
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10.1016/j.tiv.2018.06.024
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PMID:
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30003980
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In vitro aerosol exposure of epithelial cells grown at the air-liquid interface is an experimental methodology widely used in respiratory toxicology. The exposure depends to a large part on the physicochemical properties of individual aerosol constituents, as they determine the transfer kinetics from the aerosol into the cells. We characterized the transfer of 70 cigarette smoke constituents from the smoke into aqueous samples exposed in the Vitrocell® 24/48 aerosol exposure system. The amounts of these compounds in the applied smoke were determined by trapping whole smoke in N,N-dimethylformamide and then compared with their amounts in smoke-exposed, phosphate-buffered saline, yielding compound specific delivery efficiencies. Delivery efficiencies of different smoke constituents differed by up to five orders of magnitude, which indicates that the composition of the applied smoke is not necessarily representative for the delivered smoke. Therefore, dose metrics for in vitro exposure experiments should, if possible, be based on delivered and not applied doses. A comparison to literature on in vivo smoke retention in the respiratory tract indicated that the same applies for smoke retention in the respiratory tract.
(Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.)
