Ebolavirus Chimerization for the Development of a Mouse Model for Screening of Bundibugyo-Specific Antibodies.

Szczegóły
Abstrakt

Tytuł:: Ebolavirus Chimerization for the Development of a Mouse Model for Screening of Bundibugyo-Specific Antibodies.
Autorzy:: Ilinykh PA; Department of Pathology, University of Texas Medical Branch, Galveston.; Galveston National Laboratory, University of Texas Medical Branch, Galveston.
Graber J; Galveston National Laboratory, University of Texas Medical Branch, Galveston.
Kuzmina NA; Department of Pathology, University of Texas Medical Branch, Galveston.; Galveston National Laboratory, University of Texas Medical Branch, Galveston.
Huang K; Department of Pathology, University of Texas Medical Branch, Galveston.; Galveston National Laboratory, University of Texas Medical Branch, Galveston.
Ksiazek TG; Department of Pathology, University of Texas Medical Branch, Galveston.; Galveston National Laboratory, University of Texas Medical Branch, Galveston.
Crowe JE Jr; Vanderbilt Vaccine Center, Vanderbilt University Medical Center.; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center.; Chemical and Physical Biology Program, Vanderbilt University.; Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee.
Bukreyev A; Department of Pathology, University of Texas Medical Branch, Galveston.; Galveston National Laboratory, University of Texas Medical Branch, Galveston.; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston.
Źródło:: The Journal of infectious diseases [J Infect Dis] 2018 Nov 22; Vol. 218 (suppl_5), pp. S418-S422.
Typ publikacji:: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
Język:: English
Imprint Name(s):: Publication: Jan. 2011- : Oxford : Oxford University Press
Original Publication: 1904-2010 : Chicago, IL : University of Chicago Press
MeSH Terms:: Antibodies, Monoclonal/*immunology
Antibodies, Viral/*immunology
Ebolavirus/*immunology
Animals ; Antibodies, Neutralizing/immunology ; Chlorocebus aethiops ; Disease Models, Animal ; Hemorrhagic Fever, Ebola/immunology ; Mice ; Mice, Knockout ; Vero Cells ; Viral Envelope Proteins/immunology
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Grant Information:: U19 AI109711 United States AI NIAID NIH HHS
Substance Nomenclature:: 0 (Antibodies, Monoclonal)
0 (Antibodies, Neutralizing)
0 (Antibodies, Viral)
0 (Viral Envelope Proteins)
Entry Date(s):: Date Created: 20180731 Date Completed: 20190916 Latest Revision: 20200621
Update Code:: 20240104
PubMed Central ID:: PMC6249583
DOI:: 10.1093/infdis/jiy423
PMID:: 30060231
: Czasopismo naukowe

Screening of monoclonal antibodies against ebolaviruses requires small-animal models. Wild-type mice require adaptation of ebolaviruses, whereas immunodeficient mice are still resistant to nonadapted Bundibugyo ebolavirus. Swapping of Ebola virus glycoprotein with that from Bundibugyo virus resulted in a replication-competent chimeric virus, which caused 100% lethal infection in STAT1 knockout mice. Monoclonal antibody BDBV223 isolated from a human survivor of Bundibugyo virus infection protected mice from challenge with the chimeric virus. These data demonstrate the suitability of the approach for in vivo screening of antibodies and suggest the greater contribution of internal Ebola proteins in pathogenesis compared to Bundibugyo virus proteins.

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