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Tytuł pozycji:

Establishing a successful rat model of duodenal- jejunal bypass: A detailed guide.

Tytuł:
Establishing a successful rat model of duodenal- jejunal bypass: A detailed guide.
Autorzy:
Akalestou E; 1 Division of Diabetes and Nutritional Sciences, King's College London, UK.; 2 Institute of Hepatology London, Foundation for Liver Research, London, UK.; 3 Division of Transplantation, Immunology and Mucosal Biology, Faculty of Life Sciences and Medicine, King's College London, UK.
Genser L; 1 Division of Diabetes and Nutritional Sciences, King's College London, UK.; 4 Department of Surgery, King's College Hospital NHS Foundation Trust, London, UK.
Villa F; 1 Division of Diabetes and Nutritional Sciences, King's College London, UK.; 4 Department of Surgery, King's College Hospital NHS Foundation Trust, London, UK.
Christakis I; 4 Department of Surgery, King's College Hospital NHS Foundation Trust, London, UK.
Chokshi S; 2 Institute of Hepatology London, Foundation for Liver Research, London, UK.; 3 Division of Transplantation, Immunology and Mucosal Biology, Faculty of Life Sciences and Medicine, King's College London, UK.
Williams R; 2 Institute of Hepatology London, Foundation for Liver Research, London, UK.; 3 Division of Transplantation, Immunology and Mucosal Biology, Faculty of Life Sciences and Medicine, King's College London, UK.
Rubino F; 1 Division of Diabetes and Nutritional Sciences, King's College London, UK.; 4 Department of Surgery, King's College Hospital NHS Foundation Trust, London, UK.
Źródło:
Laboratory animals [Lab Anim] 2019 Aug; Vol. 53 (4), pp. 362-371. Date of Electronic Publication: 2018 Sep 18.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: Jan. 2013- : London : Sage
Original Publication: London : Laboratory Animals Ltd., 1967-
MeSH Terms:
Anastomosis, Surgical/*methods
Duodenum/*surgery
Gastric Bypass/*methods
Jejunum/*surgery
Rats/*surgery
Stomach/*surgery
Animals ; Male ; Rats, Sprague-Dawley/surgery ; Rats, Wistar/surgery
Contributed Indexing:
Keywords: bariatric surgery; duodenal-jejunal bypass; gastric bypass; metabolic surgery; obesity; type 2 diabetes mellitus
Entry Date(s):
Date Created: 20180920 Date Completed: 20191118 Latest Revision: 20191118
Update Code:
20240105
DOI:
10.1177/0023677218797370
PMID:
30227760
Czasopismo naukowe
Gastric bypass surgery, an operation that restricts the stomach and bypasses the duodenum and part of the jejunum, results in major improvement or remission of type 2 diabetes. Duodenual-jejunal bypass was developed by one of the authors (FR) as an experimental, stomach-sparing variant of gastric bypass surgery to investigate weight-independent mechanisms of surgical control of diabetes. Duodenual-jejunal bypass has been shown to improve various aspects of glucose homeostasis in rodents and in humans, thus providing an experimental model for investigating mechanisms of action of surgery and elusive aspects of gastrointestinal physiology. Performing duodenual-jejunal bypass in rodents, however, is associated with a steep learning curve. Here we report our experience with duodenual-jejunal bypass and provide practical tips for successful surgery in rats. Duodenual-jejunal bypass was performed on 50 lean rats as part of a study aimed at investigating the effect of the procedure on the physiologic mechanisms of glucose homeostasis. During the study, we have progressively refined details of anatomic exposure, technical aspects of duodeno-jejunostomy and peri-operative care. We analysed the role of such refinements in improving operative time and post-operative mortality. We found that refinement of exposure methods of the gastro-duodenal junction aimed at minimizing tension on small visceral vasculature, technical aspects of duodeno-jejunal anastomosis and peri-operative management played a major role in improving the survival rate and operative time. Overall, an experimental model of duodenual-jejunal bypass was successfully reproduced. Based on this experience, we describe here what we believe are the most important technical tips to reduce the learning curve for the procedure.

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