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Tytuł:
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Short communication: Characterization of gene expression profiles related to yak milk protein synthesis during the lactation cycle.
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Autorzy:
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Xia W; College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Conservation and Exploitation, Key Laboratory of Animal Genetics & Breeding of State Ethnic Affairs Commission and Ministry of Education, Southwest University for Nationalities, Chengdu 610041, China.
Osorio JS; Dairy and Food Science Department, South Dakota State University, Brookings 57007.
Yang Y; College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Conservation and Exploitation, Key Laboratory of Animal Genetics & Breeding of State Ethnic Affairs Commission and Ministry of Education, Southwest University for Nationalities, Chengdu 610041, China.
Liu D; 363 Hospital, Chengdu 610041, China.
Jiang MF; College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Conservation and Exploitation, Key Laboratory of Animal Genetics & Breeding of State Ethnic Affairs Commission and Ministry of Education, Southwest University for Nationalities, Chengdu 610041, China. Electronic address: .
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Źródło:
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Journal of dairy science [J Dairy Sci] 2018 Dec; Vol. 101 (12), pp. 11150-11158. Date of Electronic Publication: 2018 Sep 27.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Champaign, IL : American Dairy Science Association
Original Publication: Lancaster, Pa. [etc.]
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MeSH Terms:
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Cattle/*genetics
Cattle/*metabolism
Milk/*metabolism
Milk Proteins/*biosynthesis
AMP-Activated Protein Kinases/genetics ; AMP-Activated Protein Kinases/metabolism ; Animals ; Female ; Glucose/metabolism ; Glucose Transport Proteins, Facilitative/genetics ; Glucose Transport Proteins, Facilitative/metabolism ; Lactation ; Mammary Glands, Animal/metabolism ; Pregnancy ; Protein Biosynthesis ; Signal Transduction ; TOR Serine-Threonine Kinases/genetics ; TOR Serine-Threonine Kinases/metabolism ; Transcriptome
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Contributed Indexing:
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Keywords: amino acid and glucose transporters; mammalian target of rapamycin; protein synthesis; yak mammary
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Substance Nomenclature:
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0 (Glucose Transport Proteins, Facilitative)
0 (Milk Proteins)
EC 2.7.11.1 (TOR Serine-Threonine Kinases)
EC 2.7.11.31 (AMP-Activated Protein Kinases)
IY9XDZ35W2 (Glucose)
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Entry Date(s):
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Date Created: 20181001 Date Completed: 20190204 Latest Revision: 20211204
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Update Code:
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20240105
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DOI:
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10.3168/jds.2018-14715
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PMID:
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30268611
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This research assessed the gene expression patterns related to the synthesis of milk in yak, which is characterized by high fat and protein content but low yield. The yak (Bos grunniens) is one of the most crucial domestic animals in Tibetan life; however, the genetic and molecular factors underlying yak milk protein synthesis remain understudied. Yak mammary biopsies harvested during late-pregnancy (d -15) through the end of subsequent lactation (d 1, 15, 30, 60, 180, and 240) were used to evaluate gene expression via real-time quantitative PCR. The expression pattern of 41 genes encompassing multiple pathways integral to milk protein synthesis including insulin, mammalian target of rapamycin (mTOR), 5' AMP-activated protein kinase, Jak2-Stat5 signaling, and the expression of glucose and AA transporters was evaluated. Our results confirmed that most upregulated genes increased from d -15 and peaked at d 30 or 60 and then remained relatively highly expressed. Specifically, there was an increased expression of mTOR-related amino acid transporters (SLC1A5, SLC7A5, and SLC36A1), glucose transporters (SLC2A1, SLC2A3, and SLC2A8), Jak2-Stat5 pathway (ELF5), and insulin signaling pathway components (IRS1, PDPK1, and AKT1). For activation of proteins synthesis, MTOR was significantly increased only at d 1. Among inhibitors of mTOR signaling, TSC1 and PRKAA2 were significantly upregulated during lactation. The RPL23 was downregulated among ribosomal components. In conclusion, a critical role for AA and glucose transporters and insulin signaling through mTOR for regulation of yak milk protein synthesis was revealed in this study of the yak mammary gland.
(Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)