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Tytuł:
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Oxidative grafting vs. monolayers self-assembling on gold surface for the preparation of electrochemical immunosensors. Application to the determination of peptide YY.
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Autorzy:
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Guerrero S; Department of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, 28040 Madrid, Spain.
Agüí L; Department of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, 28040 Madrid, Spain.
Yáñez-Sedeño P; Department of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, 28040 Madrid, Spain. Electronic address: .
Pingarrón JM; Department of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, 28040 Madrid, Spain.
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Źródło:
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Talanta [Talanta] 2019 Feb 01; Vol. 193, pp. 139-145. Date of Electronic Publication: 2018 Sep 25.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
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MeSH Terms:
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Electrochemical Techniques/*methods
Gold/*chemistry
Immunoassay/*methods
Peptide YY/*blood
4-Aminobenzoic Acid/chemistry ; Alkaline Phosphatase/chemistry ; Benzoates/chemistry ; Biosensing Techniques/methods ; Calibration ; Electrodes ; Humans ; Immunoglobulin G/immunology ; Limit of Detection ; Organophosphates/chemistry ; Oxidation-Reduction ; Peptide YY/immunology ; Saliva/chemistry ; Sulfhydryl Compounds/chemistry
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Contributed Indexing:
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Keywords: Electrochemical immunosensor; Hormones; Obesity; Oxidative grafting; Peptide YY; Saliva; Serum
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Substance Nomenclature:
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0 (Benzoates)
0 (Immunoglobulin G)
0 (Organophosphates)
0 (Sulfhydryl Compounds)
106388-42-5 (Peptide YY)
1074-36-8 (4-mercaptobenzoate)
7440-57-5 (Gold)
EC 3.1.3.1 (Alkaline Phosphatase)
TL2TJE8QTX (4-Aminobenzoic Acid)
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Entry Date(s):
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Date Created: 20181029 Date Completed: 20181113 Latest Revision: 20181113
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Update Code:
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20240105
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DOI:
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10.1016/j.talanta.2018.09.089
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PMID:
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30368282
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A comparison of the performance of two electrochemical immunosensors for the determination of the anorexigen biomarker peptide YY (PYY) is reported by using as scaffolds screen printed gold electrodes modified either by oxidative grafting of p-aminobenzoic acid (p-ABA) or by assembling of a 4-mercaptobenzoic acid (4-MBA) SAM. Covalent immobilization of capture antibodies on the surface-confined carboxyl groups was carried out by EDC/NHSS chemistry, and competitive immunoassays between target PYY and Biotin-PYY were implemented. Upon labeling with alkaline phosphatase (AP)-streptavidin conjugate and 1-naphtyl phosphate addition, differential pulse voltammograms recorded between -0.2 and +0.7 V were used as analytical readout. All the steps involved in the functionalization of the electrodes and the preparation of the immunosensors were monitored by electrochemical impedance spectroscopy. The calibration plot for PYY using the AP-Strept-Biotin-PYY(PYY)-anti-PYY-Phe-N-SPAuE immunosensor provided a linear current vs. log [PYY] plot extending between 10 -6 and 10 3 ng/mL PYY with a detection limit of 3 × 10 -7 ng/mL. These analytical characteristics are remarkably better than those obtained with the immunosensor prepared with 4-MBA SAM-SPAuEs. The AP-Strept-Biotin-PYY(PYY)-anti-PYY-Phe-N-SPAuE immunosensor was used to analyze human serum and saliva samples spiked with PYY at concentrations fitting with normal levels in these biological fluids.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
