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Tytuł:
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The effect of mesenchymal stem cell-secreted factors on airway epithelial repair.
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Autorzy:
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Halim NS; Regenerative Medicine Cluster, Advanced Medical & Dental Institute (IPPT), Universiti Sains Malaysia, 13200 Bertam, Penang, Malaysia.
Aizat WM; Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Yahaya BH; Regenerative Medicine Cluster, Advanced Medical & Dental Institute (IPPT), Universiti Sains Malaysia, 13200 Bertam, Penang, Malaysia.
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Źródło:
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Regenerative medicine [Regen Med] 2019 Jan; Vol. 14 (1), pp. 15-31. Date of Electronic Publication: 2018 Dec 19.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: London, UK : Future Medicine Ltd., c2006-
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MeSH Terms:
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Epithelial Cells/*cytology
Mesenchymal Stem Cells/*metabolism
Animals ; Cell Differentiation ; Chromatography, Liquid ; Coculture Techniques ; Female ; Mesenchymal Stem Cells/cytology ; Rabbits ; Regenerative Medicine ; Tandem Mass Spectrometry
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Contributed Indexing:
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Keywords: LC–MS/MS; MSC-secreted factors; airway epithelium; airway explant; airway regeneration and repair; endogenous repair mechanism; epithelial–mesenchymal transition; human adipose-derived MSC; indirect coculture; paracrine effect
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Entry Date(s):
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Date Created: 20181220 Date Completed: 20190520 Latest Revision: 20190520
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Update Code:
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20240105
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DOI:
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10.2217/rme-2018-0020
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PMID:
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30566028
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Aim: This study was aimed to investigate the effect of mesenchymal stem cell (MSC)-secreted factors on airway repair.
Materials & Methods: An indirect in vitro coculture model of injured airway epithelium explant with MSCs was developed. LC-MS/MS analysis was performed to determine factors secreted by MSCs and their involvement in epithelium repair was evaluated by histopathological assessment.
Results: The identification of 54 of MSC proteins of which 44 of them were secretory/extracellular proteins. 43 of the secreted proteins were found to be involved in accelerating airway epithelium repair by stimulating the migratory, proliferative and differentiation abilities of the endogenous repair mechanisms. MSC-secreted proteins also initiated epithelial-mesenchymal transition process during early repair.
Conclusion: MSC-secreted factors accelerated airway epithelial repair by stimulating the endogenous reparative and regenerative ability of lung cells.
