Sunflower pan-genome analysis shows that hybridization altered gene content and disease resistance.

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Tytuł:: Sunflower pan-genome analysis shows that hybridization altered gene content and disease resistance.
Autorzy:: Hübner S; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada. .; Department of Biotechnology, Tel-Hai Academic College, Upper Galilee, Israel. .; MIGAL-Galilee Research Institute, Kiryat Shmona, Israel. .
Bercovich N; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.
Todesco M; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.
Mandel JR; Department of Biological Sciences, University of Memphis, Memphis, TN, USA.
Odenheimer J; SAP SE, Dietmar-Hopp-Allee 16, Walldorf, Germany.
Ziegler E; SAP SE, Dietmar-Hopp-Allee 16, Walldorf, Germany.
Lee JS; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.
Baute GJ; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.
Owens GL; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.; Department of Integrative Biology, University of California, Berkeley, CA, USA.
Grassa CJ; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.; Harvard University Herbaria , Cambridge, MA, USA.
Ebert DP; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.; The Beef Industry Centre, University of New England, Armidale, New South Wales, Australia.
Ostevik KL; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.; Department of Biology , Duke University, Durham, NC, USA.
Moyers BT; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.; Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO, USA.
Yakimowski S; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.
Masalia RR; Department of Plant Biology, Miller Plant Sciences, University of Georgia, Athens, Georgia, USA.
Gao L; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.
Ćalić I; Department of Plant Biology, Miller Plant Sciences, University of Georgia, Athens, Georgia, USA.
Bowers JE; Department of Plant Biology, Miller Plant Sciences, University of Georgia, Athens, Georgia, USA.
Kane NC; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.; Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, CO, USA.
Swanevelder DZH; Agricultural Research Council, Biotechnology Platform, Private Bag X05, Onderstepoort, South Africa.
Kubach T; SAP SE, Dietmar-Hopp-Allee 16, Walldorf, Germany.
Muños S; LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.
Langlade NB; LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.
Burke JM; Department of Plant Biology, Miller Plant Sciences, University of Georgia, Athens, Georgia, USA.
Rieseberg LH; Department of Botany and Biodiversity Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.
Źródło:: Nature plants [Nat Plants] 2019 Jan; Vol. 5 (1), pp. 54-62. Date of Electronic Publication: 2018 Dec 31.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
Język:: English
Imprint Name(s):: Original Publication: [London, UK] : Nature Publishing Group, a division of Macmillan Publishers Limited, [2015]-
MeSH Terms:: Hybridization, Genetic*
Helianthus/*genetics
Helianthus/*microbiology
Plant Diseases/*genetics
Crops, Agricultural/genetics ; Crops, Agricultural/microbiology ; Disease Resistance/genetics ; Gene Ontology ; Genes, Plant ; Genetic Variation ; Genome, Plant ; Plant Diseases/microbiology ; Recombination, Genetic ; Selection, Genetic
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Entry Date(s):: Date Created: 20190102 Date Completed: 20190610 Latest Revision: 20220419
Update Code:: 20240105
DOI:: 10.1038/s41477-018-0329-0
PMID:: 30598532
: Czasopismo naukowe

Domesticated plants and animals often display dramatic responses to selection, but the origins of the genetic diversity underlying these responses remain poorly understood. Despite domestication and improvement bottlenecks, the cultivated sunflower remains highly variable genetically, possibly due to hybridization with wild relatives. To characterize genetic diversity in the sunflower and to quantify contributions from wild relatives, we sequenced 287 cultivated lines, 17 Native American landraces and 189 wild accessions representing 11 compatible wild species. Cultivar sequences failing to map to the sunflower reference were assembled de novo for each genotype to determine the gene repertoire, or 'pan-genome', of the cultivated sunflower. Assembled genes were then compared to the wild species to estimate origins. Results indicate that the cultivated sunflower pan-genome comprises 61,205 genes, of which 27% vary across genotypes. Approximately 10% of the cultivated sunflower pan-genome is derived through introgression from wild sunflower species, and 1.5% of genes originated solely through introgression. Gene ontology functional analyses further indicate that genes associated with biotic resistance are over-represented among introgressed regions, an observation consistent with breeding records. Analyses of allelic variation associated with downy mildew resistance provide an example in which such introgressions have contributed to resistance to a globally challenging disease.

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