-
Tytuł:
-
Synergistic activation of Src, ERK and STAT pathways in PBMCs for Staphylococcal enterotoxin A induced production of cytokines and chemokines.
-
Autorzy:
-
Liu X; The Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, Sino-French Hoffmann Institute, Center for Immunology, Inflammation, & Immune-mediated disease, Guangzhou Medical University, Guangzhou 510260, China.
Wen Y; The Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, Sino-French Hoffmann Institute, Center for Immunology, Inflammation, & Immune-mediated disease, Guangzhou Medical University, Guangzhou 510260, China.
Wang; The Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, Sino-French Hoffmann Institute, Center for Immunology, Inflammation, & Immune-mediated disease, Guangzhou Medical University, Guangzhou 510260, China.
Zhao Z; Center for the Study of Itch, Department of Anesthesiology, Washington University School of Medicine.; Barnes-Jewish Hospital, St. Louis, MO 63110, USA.
Jeffry J; Center for the Study of Itch, Department of Anesthesiology, Washington University School of Medicine.
Zeng L; The Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, Sino-French Hoffmann Institute, Center for Immunology, Inflammation, & Immune-mediated disease, Guangzhou Medical University, Guangzhou 510260, China.
Zou Z; The Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, Sino-French Hoffmann Institute, Center for Immunology, Inflammation, & Immune-mediated disease, Guangzhou Medical University, Guangzhou 510260, China.
Chen H; The Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, Sino-French Hoffmann Institute, Center for Immunology, Inflammation, & Immune-mediated disease, Guangzhou Medical University, Guangzhou 510260, China.
Tao A; The Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, Sino-French Hoffmann Institute, Center for Immunology, Inflammation, & Immune-mediated disease, Guangzhou Medical University, Guangzhou 510260, China.
-
Źródło:
-
Asian Pacific journal of allergy and immunology [Asian Pac J Allergy Immunol] 2020 Mar; Vol. 38 (1), pp. 52-63.
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Publication: Bangkok : Allergy and Immunology Society of Thailand
Original Publication: [Bangkok : Bangkok Medical Publishers, c1983-
-
MeSH Terms:
-
Signal Transduction*
Chemokines/*immunology
Cytokines/*immunology
Enterotoxins/*immunology
Leukocytes, Mononuclear/*immunology
Adolescent ; Adult ; Cells, Cultured ; Female ; Humans ; MAP Kinase Signaling System ; Male ; Middle Aged ; Proto-Oncogene Proteins pp60(c-src)/metabolism ; STAT Transcription Factors/metabolism ; Superantigens/immunology ; Young Adult
-
Substance Nomenclature:
-
0 (Chemokines)
0 (Cytokines)
0 (Enterotoxins)
0 (STAT Transcription Factors)
0 (Superantigens)
37337-57-8 (enterotoxin A, Staphylococcal)
EC 2.7.10.2 (Proto-Oncogene Proteins pp60(c-src))
-
Entry Date(s):
-
Date Created: 20190121 Date Completed: 20200324 Latest Revision: 20200324
-
Update Code:
-
20240105
-
DOI:
-
10.12932/AP-220818-0396
-
PMID:
-
30660176
-
Background: Staphylococcal enterotoxin A (SEA) is a well-known superantigen and stimulates human peripheral blood mononuclear cells (PBMCs) involving in the pathogenesis of inflammatory disorders and cancer.
Objective: To better understand the biological activities of SEA and the possible intracellular mechanisms by which SEA plays its roles in conditions like staphylococcal inflammatory and/or autoimmune disorders and immunotherapy.
Methods: Recombinant SEA (rSEA) was expressed in a prokaryotic expression system and its effects on the cytokine and chemokine production was examined by Enzyme-linked Immunospot (ELISpot) Assay and ELISA analysis.
Results: In vitro experiments showed rSEA could significantly enhance secretion of a broad spectrum of cytokines and chemokines from PBMCs dose-dependently. Increased secretion of cytokines and chemokines from rSEA stimulated PBMCs was barely affected by C-C motif chemokine receptor 2 (CCR2) antagonist INCB3344. However, Src, ERK and STAT pathway inhibitors were able to successfully block the enhanced secretion of most of cytokines and chemokines produced by rSEA stimulated PBMCs.
Conclusions: Our work suggested that rSEA serves as a potent stimulant of PBMCs, and induces the release of cytokines and chemokines through Src, ERK and STAT pathways upon a relatively independent network. Our work also strongly supported that Src, ERK and STAT signaling inhibitors could be effective therapeutic agents against diseases like toxic shock syndrome or infection by microbes resistant to antibiotics.
