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Tytuł:
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Transcriptomic analysis of the life-extending effect exerted by black rice anthocyanin extract in D. melanogaster through regulation of aging pathways.
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Autorzy:
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Li X; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), Tianjin 300457, China; School of Marine and Biological Engineering, Yancheng Teachers University (YCTU), Yancheng 224051, China.
Zhang Z; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), Tianjin 300457, China.
Zhang X; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), Tianjin 300457, China.
Cheng J; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), Tianjin 300457, China.
Liu D; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), Tianjin 300457, China.
Yan Y; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), Tianjin 300457, China.
Wang H; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), Tianjin 300457, China. Electronic address: .
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Źródło:
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Experimental gerontology [Exp Gerontol] 2019 May; Vol. 119, pp. 33-39. Date of Electronic Publication: 2019 Jan 22.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Tarrytown Ny : Elsevier Science
Original Publication: Oxford.
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MeSH Terms:
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Anthocyanins/*pharmacology
Drosophila melanogaster/*physiology
Longevity/*drug effects
Oryza/*chemistry
Plant Extracts/*pharmacology
Seeds/*chemistry
Animals ; Animals, Genetically Modified ; Antioxidants/pharmacology ; Diet ; Drosophila Proteins/genetics ; Drosophila melanogaster/drug effects ; Drosophila melanogaster/genetics ; Forkhead Transcription Factors/genetics ; Gene Expression Profiling ; Gene Expression Regulation ; Male ; Oxidative Stress ; Signal Transduction ; Up-Regulation
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Contributed Indexing:
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Keywords: Black rice anthocyanin extract; Fruit fly; Lifespan; RNA-seq
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Substance Nomenclature:
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0 (Anthocyanins)
0 (Antioxidants)
0 (Drosophila Proteins)
0 (FOXO protein, Drosophila)
0 (Forkhead Transcription Factors)
0 (Plant Extracts)
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Entry Date(s):
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Date Created: 20190126 Date Completed: 20200715 Latest Revision: 20200715
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Update Code:
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20240105
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DOI:
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10.1016/j.exger.2019.01.015
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PMID:
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30682390
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This study examined the beneficial effects of black rice anthocyanin extract (BRAE), which provides as an excellent source of dietary antioxidants, on the lifespan of fruit flies and the underlying molecular mechanisms. Results showed that supplementation of BRAE extended the lifespan of fruit flies by 20% and retarded the loss of locomotor function. Comparison of mRNA transcriptome profile between fruit flies of 20 days and 50 days after the start of normal diet and normal diet supplemented with BRAE (5 mg/mL) identified 6687 differentially expressed coding genes. A large number of these differentially expressed mRNAs were enriched in KEGG (Kyoto Encyclopedia of genes and genomes) analysis. KEGG pathway analysis demonstrated that up regulation in the glutathione metabolism, neuroactive ligand-receptor interaction, and down regulation in FoxO signaling pathway, mTOR signaling pathway were most likely affected by supplementation of BRAE. In addition, several genes, including Zw (Zwischenferment), GstD2, GstE1, Gpx, Gclm, Tsc1, 4E-BP, DopR and D2R, which were involved in the pathways mentioned above, could be regulated by the supplementation of BRAE. Together, the results implicated that the expression of most of the mRNAs in fruit fly were closely associated with BRAE diet, which could provide a resource of mRNAs related in the field of aging and aging-related disease.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
