-
Tytuł:
-
Rapid histologic diagnosis using quick fluorescence staining and tissue confocal microscopy.
-
Autorzy:
-
Kim H; Innovative Medical Engineering & Technology, National Cancer Center, Goyang, South Korea.
Lee S; Innovative Medical Engineering & Technology, National Cancer Center, Goyang, South Korea.
Kim H; Biomarker Branch, National Cancer Center, Goyang, South Korea.
Chang HJ; Center for Colorectal Cancer, National Cancer Center, Goyang, South Korea.; Department of Pathology, National Cancer Center, Goyang, South Korea.
Choi Y; Biomarker Branch, National Cancer Center, Goyang, South Korea.
Yoon HM; Innovative Medical Engineering & Technology, National Cancer Center, Goyang, South Korea.; Center for Gastric Cancer, National Cancer Center, Goyang, South Korea.
Kook MC; Department of Pathology, National Cancer Center, Goyang, South Korea.; Center for Gastric Cancer, National Cancer Center, Goyang, South Korea.
Jung SY; Center for Breast Cancer, National Cancer Center, Goyang, South Korea.
Kwon Y; Department of Pathology, National Cancer Center, Goyang, South Korea.; Center for Breast Cancer, National Cancer Center, Goyang, South Korea.
Kang J; Department of Biomedical Engineering, Hanyang University, Seoul, South Korea.
Yoo H; Department of Biomedical Engineering, Hanyang University, Seoul, South Korea.
Song I; Nanoscope Systems, Inc., Daejeon, South Korea.
Lee S; Osong Medical Innovation Foundation, Cheongju, Chungcheongbuk-do, South Korea.
Sohn DK; Innovative Medical Engineering & Technology, National Cancer Center, Goyang, South Korea.; Center for Colorectal Cancer, National Cancer Center, Goyang, South Korea.
-
Źródło:
-
Microscopy research and technique [Microsc Res Tech] 2019 Jun; Vol. 82 (6), pp. 892-897. Date of Electronic Publication: 2019 Feb 14.
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Original Publication: New York, NY : Wiley-Liss, c1992-
-
MeSH Terms:
-
Histocytochemistry/*methods
Microscopy, Confocal/*methods
Microscopy, Fluorescence/*methods
Specimen Handling/*methods
Staining and Labeling/*methods
Benzimidazoles/metabolism ; Eosine Yellowish-(YS)/metabolism ; Humans ; Time Factors
-
Grant Information:
-
10049773 The Ministry of Trade, Industry, and Energy
-
Contributed Indexing:
-
Keywords: confocal microscopy; fluorescent image; minimally invasive surgery; quick staining method; tissue staining
-
Substance Nomenclature:
-
0 (Benzimidazoles)
P976261J69 (bisbenzimide ethoxide trihydrochloride)
TDQ283MPCW (Eosine Yellowish-(YS))
-
Entry Date(s):
-
Date Created: 20190216 Date Completed: 20190625 Latest Revision: 20190625
-
Update Code:
-
20240105
-
DOI:
-
10.1002/jemt.23234
-
PMID:
-
30767333
-
With the development of advanced and minimally invasive surgical techniques, and in view of the functional and cosmetic aspects, the need for rapid and accurate diagnosis during surgery is increasing. This study was conducted to develop a tissue diagnosis method using confocal microscopy after simple tissue staining that does not require freezing and slicing. At present, fluorescence staining with confocal microscopy is not generalized for real-time diagnosis during surgery. In this paper, we propose a fluorescence staining method using Hoechst 33342 and Eosin that does not require tissue freezing and slicing. The proposed method can be used as part of a rapid tissue diagnosis method that is suitable for use in the operating room, although further research is required before it can be applied in clinical practice.
(© 2019 Wiley Periodicals, Inc.)
