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Tytuł:
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Visualizing Embolism Propagation in Gas-Injected Leaves.
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Autorzy:
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Hochberg U; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138 .; ARO Volcani Center, Institute of Soil, Water and Environmental Sciences, Bet Dagan, 7505101 Israel.
Ponomarenko A; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138.
Zhang YJ; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138.; School of Biology and Ecology, University of Maine, Orono, Maine 04469.
Rockwell FE; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138.
Holbrook NM; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138.
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Źródło:
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Plant physiology [Plant Physiol] 2019 Jun; Vol. 180 (2), pp. 874-881. Date of Electronic Publication: 2019 Mar 06.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
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Język:
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English
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Imprint Name(s):
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Publication: : [Rockville, MD] : American Society of Plant Biologists
Original Publication: Lancaster, Pa., American Society of Plant Physiologists.
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MeSH Terms:
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Gases/*metabolism
Plant Leaves/*physiology
Xylem/*physiology
Image Processing, Computer-Assisted ; Pressure ; Quercus/physiology ; Vitis/physiology ; Water
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References:
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Substance Nomenclature:
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0 (Gases)
059QF0KO0R (Water)
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Entry Date(s):
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Date Created: 20190308 Date Completed: 20200824 Latest Revision: 20200824
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Update Code:
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20240105
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PubMed Central ID:
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PMC6548249
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DOI:
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10.1104/pp.18.01284
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PMID:
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30842264
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Because the xylem in leaves is thought to be at the greatest risk of cavitation, reliable and efficient methods to characterize leaf xylem vulnerability are of interest. We report a method to generate leaf xylem vulnerability curves (VCs) by gas injection. Using optical light transmission, we visualized embolism propagation in grapevine ( Vitis vinifera ) and red oak ( Quercus rubra ) leaves injected with positive gas pressure. This resulted in a rapid, stepwise reduction of transmitted light, identical to that observed during leaf dehydration, confirming that the optical method detects gas bubbles and provides insights into the air-seeding hypothesis. In red oak, xylem VCs generated using gas injection were similar to those generated using bench dehydration, but indicated 50% loss of conductivity at lower tension (∼0.4 MPa) in grapevine. In determining VC, this method eliminates the need to ascertain xylem tension, thus avoiding potential errors in water potential estimations. It is also much faster (1 h per VC). However, severing the petiole and applying high-pressure gas could affect air-seeding and the generated VC. We discuss potential artifacts arising from gas injection and recommend comparison of this method with a more standard procedure before it is assumed to be suitable for a given species.
(© 2019 American Society of Plant Biologists. All Rights Reserved.)
Comment in: Plant Physiol. 2019 Jun;180(2):706-707. (PMID: 31160528)