Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis.

Szczegóły
Abstrakt

Tytuł:: Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis.
Autorzy:: Nielsen AV; Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.
Woolley AT; Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA. .
Źródło:: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1972, pp. 175-184.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms:: Biomarkers/*analysis
Electrophoresis, Microchip/*instrumentation
Electrophoresis, Microchip/*methods
Fluorescent Dyes/*chemistry
Premature Birth/*diagnosis
Electrodes ; Humans ; Silicon/chemistry
References:: World Health Organization (2012) Born too soon: the global action report on preterm birth. WHO, Geneva.
March of Dimes (2013) Prematurity campaign: 2013 progress report. March of Dimes Foundation, White Plains.
Esplin MS, Merrell K, Goldenberg R et al (2011) Proteomic identification of serum peptides predicting subsequent spontaneous preterm birth. Am J Obstet Gynecol 204:391e1–391e8. (PMID: 10.1016/j.ajog.2010.09.021)
Sonker M, Knob R, Sahore V, Woolley AT (2017) Integrated electrokinetically driven microfluidic devices with pH-mediated solid-phase extraction coupled to microchip electrophoresis for preterm birth biomarkers. Electrophoresis 38:1743–1754. (PMID: 10.1002/elps.201700054)
Sahore V, Sonker M, Nielsen AV, Knob R, Kumar S, Woolley AT (2018) Automated microfluidic devices integrating solid-phase extraction, fluorescent labeling, and microchip electrophoresis for preterm birth biomarker analysis. Anal Bioanal Chem 410:933–941. (PMID: 10.1007/s00216-017-0548-7)
Sonker M, Parker EK, Nielsen AV, Sahore V, Woolley AT (2018) Electrokinetically operated microfluidic devices for integrated immunoaffinity monolith extraction and electrophoretic separation of preterm birth biomarkers. Analyst 143:224–231. (PMID: 10.1039/C7AN01357D)
Nielsen AV, Nielsen JB, Knob R, Sahore V, Woolley AT (2018) Separation of a panel of preterm birth biomarkers using microchip electrophoresis. Electrophoresis 39:2300–2307.
Beauchamp MJ, Nordin GP, Woolley AT (2017) Moving from millifluidic to truly microfluidic sub-100-μm cross-section 3D printed devices. Anal Bioanal Chem 409:4311–4319. (PMID: 10.1007/s00216-017-0398-3)
Gong H, Bickham BP, Woolley AT, Nordin GP (2017) Custom 3D printer and resin for 18 μm × 20 μm microfluidic flow channels. Lab Chip 17:2899–2909. (PMID: 10.1039/C7LC00644F)
Contributed Indexing:: Keywords: Cyclic olefin copolymer; Hot embossing; Laser induced fluorescence; Microfluidics; Photolithography; Point-of-care diagnostics; Rapid analysis
Substance Nomenclature:: 0 (Biomarkers)
0 (Fluorescent Dyes)
Z4152N8IUI (Silicon)
Entry Date(s):: Date Created: 20190309 Date Completed: 20190807 Latest Revision: 20201228
Update Code:: 20240105
DOI:: 10.1007/978-1-4939-9213-3_12
PMID:: 30847791
: Czasopismo naukowe

An unmet need exists for a clinical diagnostic to determine preterm birth (PTB) risk. Such an assessment is possible with high sensitivity and specificity using a panel of nine biomarkers. An integrated microfluidic analysis system for these biomarkers is being developed which includes microchip electrophoresis (μCE) separation. A t-shaped microchip device can be used to test the μCE portion of this integrated system to find appropriate separation conditions. These t-shaped microchips can be fabricated using photolithographically patterned Si templates and hot embossing. PTB biomarkers can be fluorescently labeled using an amine-reactive dye prior to μCE. The μCE conditions established using this t-shaped device should be useful in developing a complete integrated microfluidic system for PTB risk assessment.

Informacja