Differential gene expression profiles of human periodontal ligament cells preserved in Hank's balanced salt solution and milk.

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Tytuł:: Differential gene expression profiles of human periodontal ligament cells preserved in Hank's balanced salt solution and milk.
Autorzy:: Nam OH; Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, Korea.
Oh TJ; Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, Korea.
Lee JH; Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Korea.
Hwang YS; Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Korea.
Choi SC; Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, Korea.
Źródło:: Dental traumatology : official publication of International Association for Dental Traumatology [Dent Traumatol] 2020 Feb; Vol. 36 (1), pp. 58-68. Date of Electronic Publication: 2019 Jun 17.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Copenhagen : Munksgaard, c2001-
MeSH Terms:: Milk*
Organ Preservation Solutions*
Periodontal Ligament*/cytology
Periodontal Ligament*/metabolism
Tooth Avulsion*
Transcriptome*
Animals ; Cell Survival ; Cells, Cultured ; Humans ; Isotonic Solutions
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Grant Information:: NRF-2016R1D1A1B03931063 and MSIP&MOHW No. 2017M3A9E4048170 National Research Foundation of Korea (NRF)
Contributed Indexing:: Keywords: Hank's balanced salt solution; gene expression; milk; periodontal ligament cells
Substance Nomenclature:: 0 (Isotonic Solutions)
0 (Organ Preservation Solutions)
Entry Date(s):: Date Created: 20190504 Date Completed: 20200203 Latest Revision: 20200203
Update Code:: 20240105
DOI:: 10.1111/edt.12480
PMID:: 31050380
: Czasopismo naukowe

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Background/aim: Various types of storage media have been investigated to preserve avulsed teeth. However, the efficacies of storage media mainly focus on the aspect of cell viability. The aim of this study was to evaluate and compare the gene expression profiles of human periodontal ligament cells preserved in Hank's balanced salt solution (HBSS) and milk over different storage durations.
Material and Methods: Human periodontal ligament cells were cultured and preserved in HBSS and milk for 3 and 6 hours. Next, total RNA was isolated. QuantSeq 3' mRNA-Sequencing was used to examine differences in gene expression in HBSS- and milk-grown periodontal ligament cells. Bioinformatics analysis was also performed to predict the function of the differentially expressed genes.
Results: The number of differentially expressed genes shared among all groups was 101. In gene set enrichment analysis, the shared differentially expressed genes in HBSS and milk were associated with the TNF-α signaling pathway (P = 1.07E -7 ). Seven hallmark gene sets were also identified in HBSS. Moreover, hallmark gene sets associated with hypoxia (P = 7.26E -5 ) and apoptosis (P = 4.06E -4 ) were identified in HBSS. In milk, 10 hallmark gene sets along with gene sets for inflammatory response (P = 6.87E -3 ) were identified.
Conclusions: Compared to those in milk, genes in HBSS were differentially expressed with increasing storage duration, suggesting that diverse and different gene expression may be involved in HBSS and milk. However, a more detailed functional analysis of these differentially expressed genes in storage solutions should be performed in the future.
(© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

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