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Tytuł:
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Down-regulation of GATA1-dependent erythrocyte-related genes in the spleens of mice exposed to a space travel.
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Autorzy:
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Horie K; RIKEN Center for Integrative Medical Sciences, Yokohama, 230-0045, Japan.
Sasanuma H; Laboratory of Developmental Genetics, Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency (JAXA), Ibaraki, 305-8505, Japan.
Kudo T; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency (JAXA), Ibaraki, 305-8505, Japan.; Laboratory Animal Resource Center and Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Ibaraki, 305-8575, Japan.
Fujita SI; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency (JAXA), Ibaraki, 305-8505, Japan.; Department of Genome Biology, Faculty of Medicine, University of Tsukuba, Ibaraki, 305-8575, Japan.
Miyauchi M; RIKEN Center for Integrative Medical Sciences, Yokohama, 230-0045, Japan.
Miyao T; RIKEN Center for Integrative Medical Sciences, Yokohama, 230-0045, Japan.
Seki T; RIKEN Center for Integrative Medical Sciences, Yokohama, 230-0045, Japan.
Akiyama N; RIKEN Center for Integrative Medical Sciences, Yokohama, 230-0045, Japan.
Takakura Y; RIKEN Center for Integrative Medical Sciences, Yokohama, 230-0045, Japan.
Shimbo M; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency (JAXA), Ibaraki, 305-8505, Japan.; Laboratory Animal Resource Center and Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Ibaraki, 305-8575, Japan.
Jeon H; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency (JAXA), Ibaraki, 305-8505, Japan.; Laboratory Animal Resource Center and Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Ibaraki, 305-8575, Japan.
Shirakawa M; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency (JAXA), Ibaraki, 305-8505, Japan.; JEM Utilization Center, Human Spaceflight Technology Directorate, JAXA, Ibaraki, 305-8505, Japan.
Shiba D; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency (JAXA), Ibaraki, 305-8505, Japan.; JEM Utilization Center, Human Spaceflight Technology Directorate, JAXA, Ibaraki, 305-8505, Japan.
Yoshida N; Laboratory of Developmental Genetics, Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency (JAXA), Ibaraki, 305-8505, Japan.
Muratani M; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency (JAXA), Ibaraki, 305-8505, Japan.; Department of Genome Biology, Faculty of Medicine, University of Tsukuba, Ibaraki, 305-8575, Japan.
Takahashi S; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency (JAXA), Ibaraki, 305-8505, Japan.; Laboratory Animal Resource Center and Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Ibaraki, 305-8575, Japan.
Akiyama T; RIKEN Center for Integrative Medical Sciences, Yokohama, 230-0045, Japan. .; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency (JAXA), Ibaraki, 305-8505, Japan. .
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Źródło:
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Scientific reports [Sci Rep] 2019 May 21; Vol. 9 (1), pp. 7654. Date of Electronic Publication: 2019 May 21.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: London : Nature Publishing Group, copyright 2011-
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MeSH Terms:
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Space Flight*
Transcriptome*
GATA1 Transcription Factor/*metabolism
Spleen/*metabolism
Animals ; Down-Regulation ; Erythropoiesis ; GATA1 Transcription Factor/genetics ; Mice ; T-Cell Acute Lymphocytic Leukemia Protein 1/genetics ; T-Cell Acute Lymphocytic Leukemia Protein 1/metabolism ; Weightlessness/adverse effects
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References:
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Substance Nomenclature:
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0 (GATA1 Transcription Factor)
0 (Gata1 protein, mouse)
0 (T-Cell Acute Lymphocytic Leukemia Protein 1)
0 (Tal1 protein, mouse)
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Entry Date(s):
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Date Created: 20190523 Date Completed: 20201014 Latest Revision: 20210109
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Update Code:
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20240104
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PubMed Central ID:
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PMC6529412
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DOI:
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10.1038/s41598-019-44067-9
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PMID:
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31114014
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Secondary lymphoid organs are critical for regulating acquired immune responses. The aim of this study was to characterize the impact of spaceflight on secondary lymphoid organs at the molecular level. We analysed the spleens and lymph nodes from mice flown aboard the International Space Station (ISS) in orbit for 35 days, as part of a Japan Aerospace Exploration Agency mission. During flight, half of the mice were exposed to 1 g by centrifuging in the ISS, to provide information regarding the effect of microgravity and 1 g exposure during spaceflight. Whole-transcript cDNA sequencing (RNA-Seq) analysis of the spleen suggested that erythrocyte-related genes regulated by the transcription factor GATA1 were significantly down-regulated in ISS-flown vs. ground control mice. GATA1 and Tal1 (regulators of erythropoiesis) mRNA expression was consistently reduced by approximately half. These reductions were not completely alleviated by 1 g exposure in the ISS, suggesting that the combined effect of space environments aside from microgravity could down-regulate gene expression in the spleen. Additionally, plasma immunoglobulin concentrations were slightly altered in ISS-flown mice. Overall, our data suggest that spaceflight might disturb the homeostatic gene expression of the spleen through a combination of microgravity and other environmental changes.
