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Tytuł:
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The state-of-the-art strategies of protein engineering for enzyme stabilization.
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Autorzy:
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Liu Q; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Xun G; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Feng Y; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: .
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Źródło:
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Biotechnology advances [Biotechnol Adv] 2019 Jul - Aug; Vol. 37 (4), pp. 530-537. Date of Electronic Publication: 2018 Oct 26.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't; Review
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Język:
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English
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Imprint Name(s):
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Publication: Oxford : Elsevier Science
Original Publication: Oxford ; New York : Pergamon Press, c1983-
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MeSH Terms:
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Enzyme Stability*
Biotechnology/*trends
Enzymes/*chemistry
Protein Engineering/*trends
Biocatalysis
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Contributed Indexing:
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Keywords: Directed evolution; Enzyme stabilization; Industrial application; Protein engineering; Rational design
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Substance Nomenclature:
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0 (Enzymes)
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Entry Date(s):
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Date Created: 20190530 Date Completed: 20200123 Latest Revision: 20200123
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Update Code:
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20240104
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DOI:
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10.1016/j.biotechadv.2018.10.011
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PMID:
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31138425
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Enzymes generated by natural recruitment and protein engineering have greatly contribute in various sets of applications. However, their insufficient stability is a bottleneck that limit the rapid development of biocatalysis. Novel approaches based on precise and global structural dissection, advanced gene manipulation, and combination with the multidisciplinary techniques open a new horizon to generate stable enzymes efficiently. Here, we comprehensively introduced emerging advances of protein engineering strategies for enzyme stabilization. Then, we highlighted practical cases to show importance of enzyme stabilization in pharmaceutical and industrial applications. Combining computational enzyme design with molecular evolution will hold considerable promise in this field.
(Copyright © 2018. Published by Elsevier Inc.)
