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Tytuł:
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Determination of the magnetic properties and orientation of the heme axial ligands of PpcA from Geobacter metallireducens by paramagnetic NMR.
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Autorzy:
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Fernandes TM; UCIBIO-Requimte, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus Caparica, 2829-516 Caparica, Portugal.
Morgado L; UCIBIO-Requimte, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus Caparica, 2829-516 Caparica, Portugal.
Salgueiro CA; UCIBIO-Requimte, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus Caparica, 2829-516 Caparica, Portugal. Electronic address: .
Turner DL; Instituto de Tecnologia Química e Biológica António Xavier, Universidade NOVA de Lisboa, Avenida da República (EAN), 2780-157 Oeiras, Portugal. Electronic address: .
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Źródło:
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Journal of inorganic biochemistry [J Inorg Biochem] 2019 Sep; Vol. 198, pp. 110718. Date of Electronic Publication: 2019 May 17.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: New York, Elsevier.
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MeSH Terms:
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Bacterial Proteins/*chemistry
Cytochrome c Group/*chemistry
Geobacter/*chemistry
Heme/*chemistry
Bacterial Proteins/isolation & purification ; Cytochrome c Group/isolation & purification ; Electron Spin Resonance Spectroscopy ; Histidine/chemistry ; Ligands ; Magnetic Phenomena ; Molecular Structure
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Contributed Indexing:
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Keywords: Geobacter; Multiheme cytochrome; NMR; Paramagnetic systems
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Substance Nomenclature:
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0 (Bacterial Proteins)
0 (Cytochrome c Group)
0 (Ligands)
42VZT0U6YR (Heme)
4QD397987E (Histidine)
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SCR Organism:
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Geobacter metallireducens
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Entry Date(s):
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Date Created: 20190602 Date Completed: 20200522 Latest Revision: 20200522
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Update Code:
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20240105
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DOI:
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10.1016/j.jinorgbio.2019.110718
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PMID:
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31153111
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The rising interest in the use of Geobacter bacteria for biotechnological applications demands a deep understanding of how these bacteria are able to thrive in a variety of environments and perform extracellular electron transfer. The Geobacter metallireducens bacterium can couple the oxidation of a wide range of compounds to the reduction of several extracellular acceptors, including heavy metals, toxic organic compounds or electrode surfaces. The periplasmic c-type cytochrome PpcA from this bacterium is a member of a family composed of five periplasmic triheme cytochromes, which are important to bridge the electron transfer between the cytoplasm and the extracellular environment. To better understand the functional mechanism of PpcA it is essential to obtain structural data for this cytochrome. In this work, the geometry of the heme axial ligands, as well as the magnetic properties of the hemes were determined for the oxidized form of the cytochrome, using the 13 C NMR chemical shifts of the heme α-substituents. The results were further compared with those previously obtained for the homologous cytochrome from Geobacter sulfurreducens. The orientations of the axial histidine planes and the magnetic properties of the hemes are conserved in both proteins. Overall, the results obtained allowed the definition of the orientation of the magnetic axes of PpcA from G. metallireducens, which will be used as constraints to assist the solution structure determination of the cytochrome in the oxidized form.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
