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Tytuł pozycji:

Oligomeric proanthocyanidins and epigallocatechin gallate aggravate autophagy of foam cells through the activation of Class III PI3K/Beclin1-complex mediated cholesterol efflux.

Tytuł:
Oligomeric proanthocyanidins and epigallocatechin gallate aggravate autophagy of foam cells through the activation of Class III PI3K/Beclin1-complex mediated cholesterol efflux.
Autorzy:
Jamuna S; Department of Biochemistry, University of Madras, Chennai, Tamil Nadu, India.
Ashokkumar R; Department of Biochemistry, University of Madras, Chennai, Tamil Nadu, India.
Sakeena Sadullah MS; Department of Biochemistry, University of Madras, Chennai, Tamil Nadu, India.
Devaraj SN; Department of Biochemistry, University of Madras, Chennai, Tamil Nadu, India.
Źródło:
BioFactors (Oxford, England) [Biofactors] 2019 Sep; Vol. 45 (5), pp. 763-773. Date of Electronic Publication: 2019 Jun 25.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: Amsterdam : Ios Press
Original Publication: Oxford ; Washington, DC : Published for the International Union of Biochemistry by IRL Press, [c1988-
MeSH Terms:
Beclin-1/*genetics
Catechin/*analogs & derivatives
Cholesterol/*metabolism
Class III Phosphatidylinositol 3-Kinases/*genetics
Foam Cells/*drug effects
Proanthocyanidins/*pharmacology
ATP Binding Cassette Transporter 1/genetics ; ATP Binding Cassette Transporter 1/metabolism ; Autophagy/drug effects ; Autophagy/genetics ; Autophagy-Related Protein 5/genetics ; Autophagy-Related Protein 5/metabolism ; Beclin-1/metabolism ; Catechin/pharmacology ; Chromones/pharmacology ; Class III Phosphatidylinositol 3-Kinases/antagonists & inhibitors ; Class III Phosphatidylinositol 3-Kinases/metabolism ; Enzyme Inhibitors/pharmacology ; Foam Cells/cytology ; Foam Cells/metabolism ; Gene Expression Regulation ; Humans ; Microtubule-Associated Proteins/genetics ; Microtubule-Associated Proteins/metabolism ; Morpholines/pharmacology ; Phagosomes/drug effects ; Phagosomes/metabolism ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Proto-Oncogene Proteins c-akt/antagonists & inhibitors ; Proto-Oncogene Proteins c-akt/genetics ; Proto-Oncogene Proteins c-akt/metabolism ; Signal Transduction ; THP-1 Cells ; TOR Serine-Threonine Kinases/genetics ; TOR Serine-Threonine Kinases/metabolism
References:
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Abdul Zani I, Stephen SL, Mughal NA, Russell D, et al. Scavenger receptor structure and function in health and disease. Cell. 2015;4(2):178-201.
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Grant Information:
UGC-BSR Meritorious Fellowship University Grants Commission; UGC-UPE (Phase-II) programme [F142/2008(NS/PE)] University Grants Commission; University Grants Commission (UGC); UGC-BSR meritorious fellowship
Contributed Indexing:
Keywords: atherosclerosis; autophagy; cholesterol efflux; epigallocatechin gallate; foam cells; oligomeric proanthocyanidins
Substance Nomenclature:
0 (ABCA1 protein, human)
0 (ATP Binding Cassette Transporter 1)
0 (Autophagy-Related Protein 5)
0 (BECN1 protein, human)
0 (Beclin-1)
0 (Chromones)
0 (Enzyme Inhibitors)
0 (MAP1LC3A protein, human)
0 (Microtubule-Associated Proteins)
0 (Morpholines)
0 (Proanthocyanidins)
0 (Protein Isoforms)
31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
8R1V1STN48 (Catechin)
97C5T2UQ7J (Cholesterol)
BQM438CTEL (epigallocatechin gallate)
EC 2.7.1.1 (MTOR protein, human)
EC 2.7.1.137 (Class III Phosphatidylinositol 3-Kinases)
EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
EC 2.7.11.1 (TOR Serine-Threonine Kinases)
Entry Date(s):
Date Created: 20190626 Date Completed: 20200221 Latest Revision: 20211204
Update Code:
20240104
DOI:
10.1002/biof.1537
PMID:
31237721
Czasopismo naukowe
Foam cells are specialized types of cells which predominate the necrotic core of atherosclerotic plaque. Recently, autophagy-mediated cholesterol efflux from foam cells has been proposed as a beneficial therapy for atherosclerosis. The purpose of this study was to delineate the underlying molecular mechanism of oligomeric proanthocyanidins (OPC) and epigallocatechin gallate (EGCG) induced autophagy of foam cells and associated cholesterol efflux. The oxidized low-density lipoprotein induced foam cells demonstrated impaired autophagy flux through the downregulated expressions of LC3BII/LC3BI, autophagy related gene-5, Class III phosphoinositide 3 kinase (Class III PI3K), Beclin1, ABCA1, and ABCG1 with concomitant increase in the expressions of protein 62, Class I phosphoinositide 3 kinase, Akt, and mammalian target of rapamycin. However, these effects were significantly abolished by treatment with OPC and EGCG through activation of autophagy flux via Class III PI3K/Beclin1 and with upregulated expression of transporter proteins ABCA1 and ABCG1. Furthermore, the cholesterol efflux process in the foam cells was activated by lysosomal acid lipase and cathepsin D facilitated lipolysis of lipid droplets. Taken together, our data demonstrate that OPC and EGCG treatment stimulated the coordinated activation of autophagy and cholesterol efflux through Class III PI3K/Beclin1 pathway in foam cells, suggesting a promising therapeutic strategy against atherosclerosis.
(© 2019 International Union of Biochemistry and Molecular Biology.)
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