-
Tytuł:
-
Ficolled bone marrow is superior to bone marrow buffy coat for detection of minimal residual disease in multiple myeloma.
-
Autorzy:
-
Bai Y; a Institute for Immunology and School of Medicine , Tsinghua University , Beijing , People's Republic of China.
Chim CS; b Department of Medicine , The University of Hong Kong , Hong Kong , People's Republic of China.
-
Źródło:
-
Hematology (Amsterdam, Netherlands) [Hematology] 2019 Dec; Vol. 24 (1), pp. 533-537.
-
Typ publikacji:
-
Comparative Study; Controlled Clinical Trial; Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Publication: 2016- : Abingdon : Taylor & Francis
Original Publication: [Amsterdam] : Newark, NJ : Harwood Academic Publishers ; International Publishers Distributor,
-
MeSH Terms:
-
Ficoll*
Bone Marrow Examination/*methods
Cell Separation/*methods
Centrifugation/*methods
Multiple Myeloma/*pathology
Centrifugation, Density Gradient/methods ; Clone Cells ; DNA Primers ; DNA, Neoplasm/analysis ; DNA, Neoplasm/isolation & purification ; Gene Rearrangement ; Genes, Immunoglobulin ; Humans ; Leukocytes, Mononuclear ; Multiple Myeloma/genetics ; Neoplasm, Residual ; Neoplastic Stem Cells ; Plasma Cells ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity ; Specimen Handling
-
Contributed Indexing:
-
Keywords: ASO RQ-PCR; Minimal residual disease; bone marrow buffy coat; ficolled bone marrow; multiple myeloma; red cell lysis; sample preparation; sensitivity
-
Substance Nomenclature:
-
0 (DNA Primers)
0 (DNA, Neoplasm)
25702-74-3 (Ficoll)
-
Entry Date(s):
-
Date Created: 20190709 Date Completed: 20191216 Latest Revision: 20191217
-
Update Code:
-
20240105
-
DOI:
-
10.1080/16078454.2019.1637574
-
PMID:
-
31280705
-
Objective: Buffy coat and ficoll of bone marrow (BM) are viable options for the study of minimal residual disease (MRD) in multiple myeloma (MM). As yet, there is no data about the superiority of either sample types. Herein, we aimed to address this issue.
Methods: Forty pairs of ficolled BMs and BM buffy coats of 19 MM patients were studied for MRD by allele-specific oligonucleotide real-time quantitative PCR, with patient-specific primers/probes whenever appropriate.
Results: There were 41 pairs of MRD data for comparison analysis due to one patient with biclonal disease. MRD levels in ficolls and buffy coats were highly concordant ( r s = 0.936, P < 0.0001), with 31 (76%) and seven (17%) pairs being concomitantly MRD-positive or -negative. On the other hand, apart from the 16 pairs being both MRD-negative, or -positive but not quantifiable in ficolls and buffy coats, majority ( n = 22, 88%) had higher MRD levels in ficolled BMs than BM buffy coats. Furthermore, in 17 pairs, in which MRD was quantifiable in both, MRD levels in ficolled BMs were 3.1 times those of BM buffy coats (median, 567/10 5 vs. 184/10 5 , P = 0.001).
Conclusion: Taken together, ficolled BM is more sensitive than BM buffy coat for MRD detection in MM, hence should be recommended.
Zaloguj się, aby uzyskać dostęp do pełnego tekstu.