A Detection Method for GLUT4 Exocytosis Based on Spontaneous Split Luciferase Complementation.

Szczegóły
Abstrakt

Tytuł:: A Detection Method for GLUT4 Exocytosis Based on Spontaneous Split Luciferase Complementation.
Autorzy:: Endo M; Department of Chemistry, School of Science, The University of Tokyo.
Miyasaki M; Department of Chemistry, School of Science, The University of Tokyo.
Li Q; Department of Chemistry, School of Science, The University of Tokyo.
Kawamura G; Department of Chemistry, School of Science, The University of Tokyo.
Ozawa T; Department of Chemistry, School of Science, The University of Tokyo.
Źródło:: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2019 Aug 10; Vol. 35 (8), pp. 835-838. Date of Electronic Publication: 2019 Jul 05.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: 2022- : [Cham] : Springer
Original Publication: Tokyo : The Society, [1985-
MeSH Terms:: Exocytosis*
Genetic Complementation Test*
Glucose Transporter Type 4/*analysis
Luciferases/*metabolism
Cell Membrane/metabolism ; Glucose Transporter Type 4/metabolism ; HEK293 Cells ; High-Throughput Screening Assays ; Humans ; Luciferases/genetics
Contributed Indexing:: Keywords: GLUT4; NanoLuc; bioluminescence; exocytosis; luciferase
Substance Nomenclature:: 0 (Glucose Transporter Type 4)
0 (SLC2A4 protein, human)
EC 1.13.12.- (Luciferases)
Entry Date(s):: Date Created: 20190709 Date Completed: 20191231 Latest Revision: 20191231
Update Code:: 20240105
DOI:: 10.2116/analsci.19C003
PMID:: 31281129
: Czasopismo naukowe

Full Text Finder

Glucose transporter 4 (GLUT4) is an insulin-regulated glucose transporter, which is vital for blood glucose homeostasis. To clarify the physiological roles of GLUT4, quantitative measurement of GLUT4 exocytosis is indispensable. Herein, we show a rapid detection system for GLUT4 on the cell surface using spontaneous split-luciferase reconstitution. Upon insulin-induced GLUT4 exocytosis, GLUT4 was exposed outside, where luciferase is reconstituted and emitted luminescence. Pretreatment with inhibitors reduced the insulin-induced signal elevation. The results indicate that the developed method is applicable to high-throughput analysis on GLUT4 trafficking, which will greatly accelerate comprehensive research on the physiological roles of GLUT4.

Informacja