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Tytuł:
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Characterization of Chinese hamster ovary cells with disparate chromosome numbers: Reduction of the amount of mRNA relative to total protein.
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Autorzy:
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Yamano-Adachi N; Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: .
Ogata N; Nihon BioData Corporation, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan. Electronic address: .
Tanaka S; Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: .
Onitsuka M; Graduate School of Technology, Industrial and Social Sciences, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima, Tokushima 770-8506, Japan. Electronic address: .
Omasa T; Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: .
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Źródło:
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Journal of bioscience and bioengineering [J Biosci Bioeng] 2020 Jan; Vol. 129 (1), pp. 121-128. Date of Electronic Publication: 2019 Jul 11.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: 2003-: Osaka, Japan : Society for Biotechnology, Japan
Original Publication: Osaka, Japan : Amsterdam, The Netherlands : Society for Bioscience and Bioengineering, Japan ; Distributed outside Japan by Elsevier Science, 1999-
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MeSH Terms:
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CHO Cells/*metabolism
Chromosomes/*genetics
Immunoglobulin G/*biosynthesis
RNA, Messenger/*genetics
Animals ; CHO Cells/cytology ; Chromosomes/metabolism ; Cricetinae ; Cricetulus ; Gene Expression ; Immunoglobulin G/genetics ; RNA, Messenger/metabolism ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/genetics ; Transcriptome
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Contributed Indexing:
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Keywords: Aneuploidy; Antibody; Chinese hamster ovary cell; Chromosomal aberration; Omics
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Substance Nomenclature:
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0 (Immunoglobulin G)
0 (RNA, Messenger)
0 (Recombinant Proteins)
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Entry Date(s):
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Date Created: 20190716 Date Completed: 20200316 Latest Revision: 20200316
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Update Code:
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20240105
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DOI:
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10.1016/j.jbiosc.2019.06.012
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PMID:
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31303495
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Chromosomes in Chinese hamster ovary (CHO) cells are labile. We have shown that high-chromosome-number CHO cells have greater potential to become robust producers of recombinant proteins. One explanation being the increase in transgene integration sites. However, high-chromosome-number cell clones produce more IgG3 following culture of single-cell clones, even under conditions that yield the same number of integrations as cells with normal chromosome numbers. Here, we characterized high-chromosome-number cells by transcriptome analysis. RNA standards were used to normalize transcriptomes of cells that had different chromosome numbers. Our results demonstrate that the mRNA ratio of β-actin and many other genes in high-chromosome-number cells to that in normal-chromosome-number cells per cell (normalized to RNA standards) was smaller than the equivalent genomic size and cell volume ratios. Many genes encoding membrane proteins are more highly expressed in high-chromosome-number cells, probably due to differences in cell size caused by the increase in chromosomes. In addition, genes related to histone modification and lipid metabolism are differentially expressed. The reduced transcript level required per protein produced in total and the different intracellular signal transductions might be key factors for antibody production.
(Copyright © 2019 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)
