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Tytuł pozycji:

Soluble esterases as biomarkers of neurotoxic compounds in the widespread serpulid Ficopomatus enigmaticus (Fauvel, 1923).

Tytuł:
Soluble esterases as biomarkers of neurotoxic compounds in the widespread serpulid Ficopomatus enigmaticus (Fauvel, 1923).
Autorzy:
Casu V; Department of Veterinary Sciences, University of Pisa, San Piero a Grado (PI), Italy.
Tardelli F; Department of Veterinary Sciences, University of Pisa, San Piero a Grado (PI), Italy.
De Marchi L; Departamento de Biologia & CESAM, University of Aveiro, Aveiro, Portugal.
Monni G; Department of Veterinary Sciences, University of Pisa, San Piero a Grado (PI), Italy.
Cuccaro A; Interuniversity Center of Marine Biology (CIBM) 'G. Bacci', Leghorn, Italy.
Oliva M; Interuniversity Center of Marine Biology (CIBM) 'G. Bacci', Leghorn, Italy.
Freitas R; Departamento de Biologia & CESAM, University of Aveiro, Aveiro, Portugal.
Pretti C; Department of Veterinary Sciences, University of Pisa, San Piero a Grado (PI), Italy.; Interuniversity Center of Marine Biology (CIBM) 'G. Bacci', Leghorn, Italy.
Źródło:
Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes [J Environ Sci Health B] 2019; Vol. 54 (11), pp. 883-891. Date of Electronic Publication: 2019 Jul 16.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: 2005- : Abingdon, Oxford : Taylor & Francis
Original Publication: New York, Dekker.
MeSH Terms:
Annelida/*enzymology
Cholinesterase Inhibitors/*toxicity
Cholinesterases/*chemistry
Neurotoxins/*toxicity
Animals ; Annelida/drug effects ; Biomarkers/chemistry ; Carbamates/chemistry ; Carbaryl/chemistry ; Carbaryl/toxicity ; Carbofuran/chemistry ; Carbofuran/toxicity ; Carboxylic Ester Hydrolases/antagonists & inhibitors ; Carboxylic Ester Hydrolases/chemistry ; Carboxylic Ester Hydrolases/metabolism ; Cholinesterase Inhibitors/chemistry ; Cholinesterases/metabolism ; Kinetics ; Methomyl/chemistry ; Methomyl/toxicity ; Neurotoxins/chemistry
Contributed Indexing:
Keywords: Cholinesterase; Ficopomatus enigmaticus; Serpulidae; biomarkers; carboxylesterase
Substance Nomenclature:
0 (Biomarkers)
0 (Carbamates)
0 (Cholinesterase Inhibitors)
0 (Neurotoxins)
1NQ08HN02S (Methomyl)
EC 3.1.1.- (Carboxylic Ester Hydrolases)
EC 3.1.1.8 (Cholinesterases)
R890C8J3N1 (Carbaryl)
SKF77S6Y67 (Carbofuran)
Entry Date(s):
Date Created: 20190718 Date Completed: 20200120 Latest Revision: 20200120
Update Code:
20240105
DOI:
10.1080/03601234.2019.1640028
PMID:
31311415
Czasopismo naukowe
The characterization of soluble cholinesterases (ChEs) together with carboxylesterases (CEs) in Ficopomatus enigmaticus as suitable biomarkers of neurotoxicity was the main aim of this study. ChEs of F. enigmaticus were characterized considering enzymatic activity, substrate affinity (acetyl-, butyryl-, propionylthiocholine), kinetic parameters ( K m and V max ) and in vitro response to model inhibitors (eserine hemisulfate, iso-OMPA, BW284C51), and carbamates (carbofuran, methomyl, aldicarb, and carbaryl). CEs were characterized based on enzymatic activity, kinetic parameters and in vitro response to carbamates (carbofuran, methomyl, aldicarb, and carbaryl). Results showed that cholinesterases from F. enigmaticus showed a substrate preference for acetylthiocholine followed by propionylthiocholine; butyrylthioline was not hydrolyzed differently from other Annelida species. CE activity was in the same range of cholinesterase activity with acetylthiocholine as substrate; the enzyme activity showed high affinity for the substrate p-nytrophenyl butyrate. Carbamates inhibited ChE activity with propionylthiocholine as substrate to a higher extent than with acetylthiocoline. Also CE activity was inhibited by all tested carbamates except carbaryl. In vitro data highlighted the presence of active forms of ChEs and CEs in F. enigmaticus that could potentially be inhibited by pesticides at environmentally relevant concentration.
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