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Tytuł:
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Endometrial microbiota in infertile women with and without chronic endometritis as diagnosed using a quantitative and reference range-based method.
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Autorzy:
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Liu Y; Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China.
Ko EY; Department of Obstetrics and Gynaecology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China.
Wong KK; Department of Obstetrics and Gynaecology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China.
Chen X; Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China.
Cheung WC; Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China.
Law TS; Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China.
Chung JP; Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China.
Tsui SK; School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China; Hong Kong Bioinformatics Centre, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China.
Li TC; Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China.
Chim SS; Department of Obstetrics and Gynaecology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China. Electronic address: .
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Źródło:
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Fertility and sterility [Fertil Steril] 2019 Oct; Vol. 112 (4), pp. 707-717.e1. Date of Electronic Publication: 2019 Jul 18.
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Typ publikacji:
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Journal Article; Observational Study; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: New York. NY : Elsevier for the American Society for Reproductive Medicine
Original Publication: New York, Hoeber.
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MeSH Terms:
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Microbiota*
Bacteria/*isolation & purification
Endometritis/*microbiology
Endometrium/*microbiology
Infertility, Female/*microbiology
Case-Control Studies ; Chronic Disease ; Female ; Humans ; Reference Values
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Contributed Indexing:
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Keywords: 16S ribosomal RNA; Chronic endometritis; endometrial cavity; microbiome
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Molecular Sequence:
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ChiCTR ChiCTR-IOC-16007882
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Entry Date(s):
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Date Created: 20190723 Date Completed: 20200528 Latest Revision: 20200528
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Update Code:
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20240105
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DOI:
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10.1016/j.fertnstert.2019.05.015
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PMID:
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31327470
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Objective: To systematically compare the endometrial microbiota in infertile women with and without chronic endometritis (CE), as diagnosed by a quantitative and reference range-based method.
Design: Case-control observational study.
Setting: University-affiliated hospital.
Patient(s): One hundred and thirty infertile women.
Intervention(s): Endometrial biopsy and fluid (uterine lavage, UL) collected precisely 7 days after LH surge, with plasma cell density (PCD) determined based on Syndecan-1 (CD138)-positive cells in the entire biopsy section and culture-independent massively parallel sequencing of the 16S ribosomal RNA gene performed on both the CE and non-CE endometrial fluid samples.
Main Outcome Measure(s): Relative abundance of bacterial taxa.
Result(s): Chronic endometritis was diagnosed if the PCD was above the 95th percentile (>5.15 cells per 10 mm 2 ) of the reference range in fertile control subjects. With this stringent diagnostic criterion, 12 women (9%) were diagnosed with CE. Sequencing was successfully performed on all endometrial samples obtained by UL) (CE, n = 12; non-CE, n = 118). The median relative abundance of Lactobacillus was 1.89% and 80.7% in the CE and non-CE microbiotas, respectively. Lactobacillus crispatus was less abundant in the CE microbiota (fold-change, range: 2.10-2.30). Eighteen non-Lactobacillus taxa including Dialister, Bifidobacterium, Prevotella, Gardnerella, and Anaerococcus were more abundant in the CE microbiota (fold-change, 2.10-18.9). Of these, Anaerococcus and Gardnerella were negatively correlated in relative abundance with Lactobacillus (SparCC correlation magnitude, range: 0.142-0.177).
Conclusion(s): Chronic endometritis was associated with a statistically significantly higher abundance of 18 bacterial taxa in the endometrial cavity.
Clinical Trials Registry Number: ChiCTR-IOC-16007882.
(Copyright © 2019 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.)
Comment in: Fertil Steril. 2019 Oct;112(4):649-650. (PMID: 31371046)
