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Tytuł:
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Screening of immuno-modulatory potential of different herbal plant extracts using striped catfish (Pangasianodon hypophthalmus) leukocyte-based in vitro tests.
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Autorzy:
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Nhu TQ; Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth and Environment (ILEE), University of Namur, Rue de Bruxelles 61, B-5000, Namur, Belgium; College of Aquaculture and Fisheries, Cantho University, Campus II, Cantho City, Viet Nam. Electronic address: .
Bich Hang BT; College of Aquaculture and Fisheries, Cantho University, Campus II, Cantho City, Viet Nam. Electronic address: .
Vinikas A; Pharmacognosy Research Group, Louvain Drug Research Institute, Université Catholique de Louvain, Avenue E. Mounier, 72 bte B1. 72.03, Brussels, Belgium. Electronic address: .
Bach LT; College of Natural Sciences, Cantho University, Campus II, Cantho City, Viet Nam. Electronic address: .
Buu Hue BT; College of Natural Sciences, Cantho University, Campus II, Cantho City, Viet Nam. Electronic address: .
Thanh Huong DT; College of Aquaculture and Fisheries, Cantho University, Campus II, Cantho City, Viet Nam. Electronic address: .
Quetin-Leclercq J; Pharmacognosy Research Group, Louvain Drug Research Institute, Université Catholique de Louvain, Avenue E. Mounier, 72 bte B1. 72.03, Brussels, Belgium. Electronic address: .
Scippo ML; Department of Food Sciences, Laboratory of Food Analysis, Faculty of Veterinary Medicine, Fundamental and Applied Research for Animals & Health (FARAH), Veterinary Public Health, University of Liège, bât. B43bis, 10 Avenue de Cureghem, Sart-Tilman, Liège, Belgium. Electronic address: .
Phuong NT; College of Aquaculture and Fisheries, Cantho University, Campus II, Cantho City, Viet Nam. Electronic address: .
Kestemont P; Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth and Environment (ILEE), University of Namur, Rue de Bruxelles 61, B-5000, Namur, Belgium. Electronic address: .
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Źródło:
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Fish & shellfish immunology [Fish Shellfish Immunol] 2019 Oct; Vol. 93, pp. 296-307. Date of Electronic Publication: 2019 Jul 25.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: London ; New York : Academic Press, c1991-
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MeSH Terms:
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Immunomodulation*
Catfishes/*immunology
Immunologic Factors/*pharmacology
Leukocytes/*immunology
Plant Extracts/*pharmacology
Animals ; Head Kidney/drug effects ; Head Kidney/immunology ; Immunologic Factors/administration & dosage ; Leukocytes/drug effects ; Leukocytes, Mononuclear/drug effects ; Leukocytes, Mononuclear/immunology ; Plant Extracts/administration & dosage ; Species Specificity
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Contributed Indexing:
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Keywords: Ethanol plant extracts; Head kidney leukocytes; Immune system; Pangasianodon hypophthalmus; Peripheral blood mononuclear cells; Striped catfish
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Substance Nomenclature:
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0 (Immunologic Factors)
0 (Plant Extracts)
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Entry Date(s):
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Date Created: 20190729 Date Completed: 20200106 Latest Revision: 20200106
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Update Code:
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20240105
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DOI:
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10.1016/j.fsi.2019.07.064
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PMID:
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31352112
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Many medicinal plants have been shown to possess biological effects, including immuno-modulatory activities on human and other mammals. However, studies about the potential mechanisms of plant extracts on the humoral and tissular immunities in fish have received less attention. This study aimed to screen the immunestimulating properties of 20 ethanol plant extracts on striped catfish Pangasianodon hypophthalmus leukocytes. The peripheral blood mononuclear cells (PBMCs) and head kidney leukocytes (HKLs) of striped catfish (50 ± 5 g per fish) were stimulated at 10 and 100 μg of each plant extract per mL of cell culture medium. Several humoral immune parameters (lysozyme, complement and total immunoglobulin) were examined at 24-h post stimulation (hps). Furthermore, the responses of four cytokine genes, namely il1β, ifrγ 2a and b, and mhc class II were assessed by quantitative real-time PCR at 6, 12, 24, and 48 hps. The results showed that lysozyme, complement as well as total immunoglobulin levels in both PBMCs and HKLs were regulated by some of the plant extracts tested in a concentration-dependent manner; some plant extracts induced the highest immune responses at the low dose (10 μg mL -1 ) while others were more efficient at high dose (100 μg mL -1 ). Among the extracts, five extracts including garlic Allium sativum L. (As), neem Azadirachta indica A. Juss (Ai), asthma-plant Euphorbia hirta L. (Eh), bhumi amla Phyllanthus amarus Schum. et Thonn (Pa), and ginger Zingiber officinale Rosc (Zo) induced significant changes in the expression of pro-inflammatory cytokine (il1β), antiviral cytokines (ifrγ 2a and b) and adaptive immune cytokine (mhc class II) in striped catfish cells. Pa always modulated the strongest expression of the four cytokines in PBMCs and HKLs over the whole experimental period (p < 0.05), whereas Zo did not stimulate the mhc class II expression in striped catfish leukocytes throughout experimental periods. These in vitro results demonstrated that some plant extracts could differently modulate great potential immune response in fish, supporting their applications in further in vivo experiments.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
