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Tytuł:
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A rapid diagnostic multiplex PCR approach for xenomonitoring of human and animal schistosomiasis in a 'One Health' context.
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Autorzy:
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Schols R; Laboratory of Biodiversity and Evolutionary Genomics, University of Leuven, Leuven, Belgium.
Carolus H; Laboratory of Biodiversity and Evolutionary Genomics, University of Leuven, Leuven, Belgium.
Hammoud C; Department of Biology, Royal Museum for Central Africa, Leuvensesteenweg 13, Tervuren, Belgium.; Limnology Research Unit, Ghent University, K. L. Ledeganckstraat 35, Ghent, Belgium.
Mulero S; Laboratory of Host-Pathogen-Environment Interactions, IHPE UMR 5244, CNRS, University of Perpignan Via Domitia, Perpignan, France.
Mudavanhu A; Department of Biological Sciences, University of Zimbabwe, Harare, Zimbabwe.
Huyse T; Laboratory of Biodiversity and Evolutionary Genomics, University of Leuven, Leuven, Belgium.; Department of Biology, Royal Museum for Central Africa, Leuvensesteenweg 13, Tervuren, Belgium.
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Źródło:
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Transactions of the Royal Society of Tropical Medicine and Hygiene [Trans R Soc Trop Med Hyg] 2019 Nov 01; Vol. 113 (11), pp. 722-729.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: 2013- : Oxford : Oxford University Press
Original Publication: 1920- : London : Royal Society of Tropical Medicine and Hygiene
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MeSH Terms:
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Species Specificity*
Multiplex Polymerase Chain Reaction/*methods
One Health/*standards
RNA, Protozoan/*genetics
Schistosoma/*genetics
Schistosomiasis/*diagnosis
Schistosomiasis/*veterinary
Animals ; Genetic Variation ; Humans ; One Health/statistics & numerical data ; Parasitic Diseases, Animal/epidemiology ; Parasitic Diseases, Animal/genetics ; Schistosomiasis/epidemiology ; Sensitivity and Specificity ; South Africa ; Zimbabwe/epidemiology
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Contributed Indexing:
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Keywords: Schistosoma; One Health; Trematoda; gastropod-borne disease; rapid diagnostic multiplex PCR; transmission monitoring
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Substance Nomenclature:
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0 (RNA, Protozoan)
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Entry Date(s):
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Date Created: 20190802 Date Completed: 20200921 Latest Revision: 20200921
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Update Code:
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20240105
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DOI:
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10.1093/trstmh/trz067
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PMID:
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31369105
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Studying the epidemiology of schistosomiasis-the most prevalent gastropod-borne human disease and an economic burden for the livestock industry-relies on adequate monitoring tools. Here we describe a molecular assay for detecting human and animal African schistosome species in their planorbid gastropod host (xenomonitoring) using a two-step approach. First, schistosome infections are detected and discriminated from other trematode infections using a multiplex polymerase chain reaction (PCR) that includes a trematode-specific marker (in 18S rDNA), a Schistosoma genus-specific marker (in internal transcribed spacer 2 [ITS2]) and a general gastropod marker (in 18S rDNA) as an internal control. Upon Schistosoma sp. detection, a second multiplex PCR is performed to discriminate among Schistosoma haematobium, Schistosoma mansoni, Schistosoma mattheei and Schistosoma bovis/Schistosoma curassoni/Schistosoma guineensis using markers of differential lengths in the cytochrome c oxidase subunit 1 (COX1) gene. The specificity of these assays was validated with adult worms, naturally infected gastropods and human urine and stool samples. Sensitivity was tested on experimentally infected snail specimens that were sacrificed 10 and 40 days post-infection in order to mimic a natural prepatent and mature infection, respectively. The assay provides a diagnostic tool to support the xenomonitoring of planorbid gastropods for trematode infections in a One Health context, with a focus on the transmission monitoring of schistosomiasis.
(© The Author(s) 2019. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
