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Tytuł pozycji:

In Vivo Imaging of Cerebrospinal Fluid Transport through the Intact Mouse Skull using Fluorescence Macroscopy.

Tytuł:
In Vivo Imaging of Cerebrospinal Fluid Transport through the Intact Mouse Skull using Fluorescence Macroscopy.
Autorzy:
Sweeney AM; Center for Translational Neuromedicine, University of Rochester Medical Center.
Plá V; Center for Translational Neuromedicine, University of Rochester Medical Center.
Du T; Center for Translational Neuromedicine, University of Rochester Medical Center.
Liu G; Center for Translational Neuromedicine, University of Rochester Medical Center.
Sun Q; Center for Translational Neuromedicine, University of Rochester Medical Center.
Peng S; Center for Translational Neuromedicine, University of Rochester Medical Center.
Plog BA; Center for Translational Neuromedicine, University of Rochester Medical Center.
Kress BT; Center for Translational Neuromedicine, University of Rochester Medical Center.
Wang X; Center for Translational Neuromedicine, University of Rochester Medical Center.
Mestre H; Center for Translational Neuromedicine, University of Rochester Medical Center; Humberto_.
Nedergaard M; Center for Translational Neuromedicine, University of Rochester Medical Center; Center for Translational Neuromedicine, University of Copenhagen; maiken_.
Źródło:
Journal of visualized experiments : JoVE [J Vis Exp] 2019 Jul 29 (149). Date of Electronic Publication: 2019 Jul 29.
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Video-Audio Media
Język:
English
Imprint Name(s):
Original Publication: [Boston, Mass. : MYJoVE Corporation, 2006]-
MeSH Terms:
Brain/*physiology
Cerebrospinal Fluid/*physiology
Glymphatic System/*physiology
Imaging, Three-Dimensional/*instrumentation
Animals ; Brain/diagnostic imaging ; Cerebrospinal Fluid/diagnostic imaging ; Glymphatic System/diagnostic imaging ; Imaging, Three-Dimensional/methods ; Mice ; Microscopy, Fluorescence ; Skull
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Grant Information:
R01 NS100366 United States NS NINDS NIH HHS; RF1 AG057575 United States AG NIA NIH HHS; RF1 NS110049 United States NS NINDS NIH HHS
Entry Date(s):
Date Created: 20190813 Date Completed: 20200615 Latest Revision: 20200615
Update Code:
20240104
PubMed Central ID:
PMC7001880
DOI:
10.3791/59774
PMID:
31403617
Czasopismo naukowe
Cerebrospinal fluid (CSF) flow in rodents has largely been studied using ex vivo quantification of tracers. Techniques such as two-photon microscopy and magnetic resonance imaging (MRI) have enabled in vivo quantification of CSF flow but they are limited by reduced imaging volumes and low spatial resolution, respectively. Recent work has found that CSF enters the brain parenchyma through a network of perivascular spaces surrounding the pial and penetrating arteries of the rodent cortex. This perivascular entry of CSF is a primary driver of the glymphatic system, a pathway implicated in the clearance of toxic metabolic solutes (e.g., amyloid-β). Here, we illustrate a new macroscopic imaging technique that allows real-time, mesoscopic imaging of fluorescent CSF tracers through the intact skull of live mice. This minimally-invasive method facilitates a multitude of experimental designs and enables single or repeated testing of CSF dynamics. Macroscopes have high spatial and temporal resolution and their large gantry and working distance allow for imaging while performing tasks on behavioral devices. This imaging approach has been validated using two-photon imaging and fluorescence measurements obtained from this technique strongly correlate with ex vivo fluorescence and quantification of radio-labeled tracers. In this protocol, we describe how transcranial macroscopic imaging can be used to evaluate glymphatic transport in live mice, offering an accessible alternative to more costly imaging modalities.

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