Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

Regulation of gene expression by altered promoter methylation using a CRISPR/Cas9-mediated epigenetic editing system.

Tytuł:
Regulation of gene expression by altered promoter methylation using a CRISPR/Cas9-mediated epigenetic editing system.
Autorzy:
Kang JG; Genome Editing Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Korea.
Park JS; Genome Editing Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Korea.; Department of Biomolecular Science, KRIBB School of Bioscience, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Korea.
Ko JH; Genome Editing Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Korea. .; Department of Biomolecular Science, KRIBB School of Bioscience, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Korea. .
Kim YS; Genome Editing Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Korea. .; Department of Biomolecular Science, KRIBB School of Bioscience, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Korea. .
Źródło:
Scientific reports [Sci Rep] 2019 Aug 19; Vol. 9 (1), pp. 11960. Date of Electronic Publication: 2019 Aug 19.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:
CRISPR-Cas Systems*
DNA Methylation*
Epigenesis, Genetic*
Gene Editing*
Gene Expression Regulation*
Promoter Regions, Genetic*
Animals ; Cloning, Molecular ; CpG Islands ; Gene Knock-In Techniques ; Gene Targeting ; Histones/metabolism ; Mice ; NIH 3T3 Cells ; Octamer Transcription Factor-3/chemistry ; Plasmids/genetics ; RNA, Guide, CRISPR-Cas Systems
References:
Nat Rev Genet. 2009 May;10(5):295-304. (PMID: 19308066)
J Biol Chem. 2004 Apr 23;279(17):17063-9. (PMID: 14761969)
Cell Rep. 2015 Aug 18;12(7):1184-95. (PMID: 26257180)
Curr Opin Cell Biol. 2001 Jun;13(3):263-73. (PMID: 11343896)
Transl Cancer Res. 2013 Jun;2(3):130-143. (PMID: 24000320)
Cell Discov. 2016 May 03;2:16009. (PMID: 27462456)
Sci Rep. 2015 Oct 07;5:14410. (PMID: 26442875)
Sci Rep. 2014 Jun 23;4:5396. (PMID: 24953798)
Adv Genet. 2010;70:27-56. (PMID: 20920744)
Cell. 2009 Feb 6;136(3):411-9. (PMID: 19203577)
Biol Open. 2016 Jun 15;5(6):866-74. (PMID: 27170255)
J Histochem Cytochem. 2013 Apr;61(4):306-12. (PMID: 23321776)
DNA Cell Biol. 2017 Sep;36(9):725-736. (PMID: 28731785)
Nat Methods. 2016 Jul;13(7):563-567. (PMID: 27214048)
Cell. 2016 Sep 22;167(1):233-247.e17. (PMID: 27662091)
Elife. 2016 Apr 28;5:. (PMID: 27130520)
Nucleic Acids Res. 2016 May 19;44(9):e85. (PMID: 26850641)
Oncol Rep. 2013 Jul;30(1):201-6. (PMID: 23670345)
Genes Dev. 2011 May 15;25(10):1010-22. (PMID: 21576262)
Nature. 2007 May 24;447(7143):425-32. (PMID: 17522676)
FEBS Lett. 2009 Jun 5;583(11):1713-20. (PMID: 19376112)
Nat Rev Genet. 2016 Aug;17(8):487-500. (PMID: 27346641)
Genome Res. 2014 Jun;24(6):1012-9. (PMID: 24696461)
Science. 2010 Jan 8;327(5962):167-70. (PMID: 20056882)
Mamm Genome. 2001 Apr;12(4):309-17. (PMID: 11309664)
Nat Methods. 2016 Dec;13(12):1043-1049. (PMID: 27776111)
Nat Biotechnol. 2014 Jul;32(7):670-6. (PMID: 24752079)
Nat Rev Genet. 2012 Jan 04;13(2):97-109. (PMID: 22215131)
Int J Cancer. 2008 Jul 1;123(1):8-13. (PMID: 18425818)
Cell. 2007 Feb 23;128(4):635-8. (PMID: 17320500)
Science. 2009 May 15;324(5929):930-5. (PMID: 19372391)
Nucleic Acids Res. 2013 Mar 1;41(5):2918-31. (PMID: 23355616)
Epigenetics Chromatin. 2017 May 8;10:24. (PMID: 28503202)
Nat Genet. 2003 Mar;33 Suppl:245-54. (PMID: 12610534)
PLoS One. 2010 Apr 01;5(4):e9937. (PMID: 20376339)
Methods. 2019 Jul 15;164-165:109-119. (PMID: 31071448)
Clin Immunol. 2003 Oct;109(1):103-8. (PMID: 14585281)
Cell Stem Cell. 2008 Feb 7;2(2):160-9. (PMID: 18371437)
Genome Res. 2014 Jan;24(1):142-53. (PMID: 24179142)
Nat Commun. 2018 Jul 6;9(1):2643. (PMID: 29980666)
Nat Rev Genet. 2015 Feb;16(2):71-84. (PMID: 25554358)
J Pathol. 2001 Sep;195(1):97-110. (PMID: 11568896)
Nat Protoc. 2013 Nov;8(11):2281-2308. (PMID: 24157548)
Stem Cells. 2007 Feb;25(2):500-10. (PMID: 17068183)
Nat Rev Genet. 2012 May 29;13(7):484-92. (PMID: 22641018)
Oncogene. 2008 Feb 28;27(10):1376-86. (PMID: 17828306)
Mol Cell Biol. 2008 Jan;28(2):752-71. (PMID: 17991895)
Oncotarget. 2016 Jul 19;7(29):46545-46556. (PMID: 27356740)
Nat Genet. 2010 Dec;42(12):1093-100. (PMID: 21057502)
Cell. 2018 Feb 22;172(5):979-992.e6. (PMID: 29456084)
Cell Res. 2008 Jan;18(1):99-113. (PMID: 18166982)
Substance Nomenclature:
0 (Histones)
0 (Octamer Transcription Factor-3)
0 (Pou5f1 protein, mouse)
0 (RNA, Guide, CRISPR-Cas Systems)
Entry Date(s):
Date Created: 20190821 Date Completed: 20201102 Latest Revision: 20240104
Update Code:
20240104
PubMed Central ID:
PMC6700181
DOI:
10.1038/s41598-019-48130-3
PMID:
31427598
Czasopismo naukowe
Despite the increased interest in epigenetic research, its progress has been hampered by a lack of satisfactory tools to control epigenetic factors in specific genomic regions. Until now, many attempts to manipulate DNA methylation have been made using drugs but these drugs are not target-specific and have global effects on the whole genome. However, due to new genome editing technologies, potential epigenetic factors can now possibly be regulated in a site-specific manner. Here, we demonstrate the utility of CRISPR/Cas9 to modulate methylation at specific CpG sites and to elicit gene expression. We targeted the murine Oct4 gene which is transcriptionally locked due to hypermethylation at the promoter region in NIH3T3 cells. To induce site-specific demethylation at the Oct4 promoter region and its gene expression, we used the CRISPR/Cas9 knock-in and CRISPR/dCas9-Tet1 systems. Using these two approaches, we induced site-specific demethylation at the Oct4 promoter and confirmed the up-regulation of Oct4 expression. Furthermore, we confirmed that the synergistic effect of DNA demethylation and other epigenetic regulations increased the expression of Oct4 significantly. Based on our research, we suggest that our proven epigenetic editing methods can selectively modulate epigenetic factors such as DNA methylation and have promise for various applications in epigenetics.

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies