Direct electrical stimulation enhances osteogenesis by inducing Bmp2 and Spp1 expressions from macrophages and preosteoblasts.

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Abstrakt

Tytuł:: Direct electrical stimulation enhances osteogenesis by inducing Bmp2 and Spp1 expressions from macrophages and preosteoblasts.
Autorzy:: Srirussamee K; Department of Materials, The University of Manchester, Manchester, UK.
Mobini S; Instituto de Micro y Nanotecnología IMN-CNM, The Spanish National Research Council (CSIC), Madrid, Spain.; Departamento de Biología Molecular and Centro de Biología Molecular 'Severo Ochoa' (UAM-CSIC), Universidad Autónoma de Madrid, Madrid, Spain.
Cassidy NJ; Department of Civil Engineering, University of Birmingham, Birmingham, UK.
Cartmell SH; Department of Materials, The University of Manchester, Manchester, UK.
Źródło:: Biotechnology and bioengineering [Biotechnol Bioeng] 2019 Dec; Vol. 116 (12), pp. 3421-3432. Date of Electronic Publication: 2019 Sep 23.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: <2005->: Hoboken, NJ : Wiley
Original Publication: New York, Wiley.
MeSH Terms:: Gene Expression Regulation*
Osteogenesis*
Bone Morphogenetic Protein 2/*biosynthesis
Macrophages/*metabolism
Osteoblasts/*metabolism
Osteopontin/*biosynthesis
Animals ; Cell Line ; Coculture Techniques ; Electric Stimulation ; Macrophages/cytology ; Mice ; Osteoblasts/cytology
References:: Biochem Biophys Res Commun. 1998 Sep 18;250(2):458-61. (PMID: 9753652)
Biochim Biophys Acta. 2006 Sep;1763(9):907-16. (PMID: 16930744)
J Biomed Mater Res B Appl Biomater. 2019 May;107(4):924-932. (PMID: 30265775)
Adv Wound Care (New Rochelle). 2014 Feb 1;3(2):91-97. (PMID: 24761349)
J Neurosci Methods. 2005 Feb 15;141(2):171-98. (PMID: 15661300)
J Bone Joint Surg Am. 2001 Oct;83(10):1514-23. (PMID: 11679602)
J Cell Commun Signal. 2009 Dec;3(3-4):311-22. (PMID: 19798593)
Mediators Inflamm. 2015;2015:816460. (PMID: 26089604)
Biochem Biophys Res Commun. 2010 Jun 18;397(1):12-7. (PMID: 20452327)
J Craniomaxillofac Surg. 2016 Jun;44(6):715-24. (PMID: 27085985)
Anat Rec (Hoboken). 2009 Aug;292(8):1107-13. (PMID: 19645015)
Biochem Biophys Res Commun. 1997 Aug 18;237(2):225-9. (PMID: 9268690)
Nature. 1964 Nov 14;204:652-4. (PMID: 14236279)
Biochem Pharmacol. 2009 Apr 15;77(8):1303-15. (PMID: 19071092)
Clin Orthop Relat Res. 1977 May;(124):5-8. (PMID: 340088)
IEEE Rev Biomed Eng. 2018;11:217-232. (PMID: 29994564)
Am J Physiol Heart Circ Physiol. 2001 May;280(5):H2357-63. (PMID: 11299242)
PLoS One. 2013 Sep 11;8(9):e72978. (PMID: 24039834)
IUBMB Life. 2007 Jan;59(1):27-33. (PMID: 17365177)
Anat Rec. 1996 Jun;245(2):394-409. (PMID: 8769675)
Acta Biomater. 2018 Apr 15;71:37-48. (PMID: 29505890)
Circ Res. 2005 Apr 15;96(7):e59-67. (PMID: 15790955)
Biotechniques. 2016 Feb 01;60(2):95-8. (PMID: 26842356)
Biomed Microdevices. 2015 Feb;17(1):24. (PMID: 25663443)
J Immunol. 2011 Mar 1;186(5):3173-9. (PMID: 21257959)
J Bone Miner Res. 2015 Jun;30(6):1090-102. (PMID: 25487241)
Biochem Biophys Rep. 2017 Feb 27;10:17-25. (PMID: 28955732)
J Biomed Mater Res B Appl Biomater. 2019 Jul;107(5):1607-1619. (PMID: 30318825)
Spine (Phila Pa 1976). 2007 Jan 15;32(2):174-81. (PMID: 17224811)
Exp Cell Res. 2007 Apr 1;313(6):1149-60. (PMID: 17306792)
Acta Biomater. 2016 Mar 1;32:46-56. (PMID: 26703122)
Nat Genet. 2006 Dec;38(12):1424-9. (PMID: 17099713)
Annu Rev Physiol. 2017 Feb 10;79:541-566. (PMID: 27813830)
J Biomed Mater Res A. 2009 Mar 15;88(4):858-71. (PMID: 18357567)
Antioxid Redox Signal. 2006 Mar-Apr;8(3-4):243-70. (PMID: 16677071)
Stem Cells Dev. 2008 Aug;17(4):805-13. (PMID: 18715162)
Biochem Biophys Res Commun. 2006 Apr 14;342(3):902-8. (PMID: 16500625)
J Biol Chem. 2014 Jan 3;289(1):275-85. (PMID: 24247243)
Eplasty. 2011;11:e34. (PMID: 21847434)
Med Biol Eng Comput. 1979 Nov;17(6):769-75. (PMID: 317920)
Dev Biol. 1994 Jan;161(1):218-28. (PMID: 8293874)
Exp Mol Med. 2013 Jan 18;45:e6. (PMID: 23306704)
Tissue Eng Part B Rev. 2013 Feb;19(1):48-57. (PMID: 22873689)
J Leukoc Biol. 2016 Jun;99(6):1141-51. (PMID: 26718542)
Mol Reprod Dev. 1992 Jun;32(2):160-7. (PMID: 1637554)
Adv Exp Med Biol. 2010;658:43-9. (PMID: 19950014)
Biomaterials. 2007 Aug;28(22):3263-72. (PMID: 17466367)
Clin Orthop Relat Res. 1981 Nov-Dec;(161):122-32. (PMID: 7307377)
Bone. 2002 Jan;30(1):26-31. (PMID: 11792561)
J Clin Invest. 2003 Nov;112(9):1318-31. (PMID: 14597759)
Clin Orthop Relat Res. 1975;(107):277-82. (PMID: 236849)
Med Sci Monit Basic Res. 2016 Sep 26;22:95-106. (PMID: 27667570)
Bone Miner. 1994 Jan;24(1):59-73. (PMID: 8186734)
Stem Cells. 2014 Feb;32(2):327-37. (PMID: 24123709)
Cell Res. 2013 Jul;23(7):898-914. (PMID: 23752925)
Inflamm Res. 2012 Dec;61(12):1329-38. (PMID: 22842767)
Indian J Orthop. 2009 Apr;43(2):127-31. (PMID: 19838360)
Endocrinology. 2000 Nov;141(11):3956-64. (PMID: 11089525)
Integr Biol (Camb). 2014 Sep;6(9):817-30. (PMID: 25058796)
Sci Rep. 2016 Aug 19;6:31724. (PMID: 27539550)
J Bone Joint Surg Am. 2008 Nov;90(11):2322-30. (PMID: 18978400)
Eur Spine J. 2006 Sep;15(9):1301-11. (PMID: 16604354)
J Vis Exp. 2019 Jan 31;(143):. (PMID: 30774122)
J Cell Biol. 2016 Apr 25;213(2):275-88. (PMID: 27091452)
JBJS Rev. 2017 Aug;5(8):e8. (PMID: 28806266)
Biotechnol Bioeng. 2019 Dec;116(12):3421-3432. (PMID: 31429922)
Tissue Eng Part A. 2010 Mar;16(3):917-31. (PMID: 19824802)
Sci Rep. 2018 May 25;8(1):8171. (PMID: 29802255)
Bone. 2018 Jan;106:78-89. (PMID: 26529389)
J Leukoc Biol. 1996 Oct;60(4):540-5. (PMID: 8864140)
FASEB J. 2007 May;21(7):1472-80. (PMID: 17264165)
FASEB J. 2004 Dec;18(15):1903-5. (PMID: 15385433)
J Bone Joint Surg Am. 2006 May;88(5):1053-65. (PMID: 16651580)
Biomaterials. 2018 Jan;150:60-86. (PMID: 29032331)
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:6517-21. (PMID: 19964171)
Int J Mol Med. 2017 Aug;40(2):281-292. (PMID: 28627621)
Sci Rep. 2018 Apr 20;8(1):6307. (PMID: 29679025)
Spine J. 2019 Jun;19(6):1106-1120. (PMID: 30584910)
J Orthop Res. 1983;1(2):120-8. (PMID: 6679856)
Apoptosis. 2015 Mar;20(3):273-84. (PMID: 25633408)
Biochem Biophys Res Commun. 1999 Nov 2;264(3):657-61. (PMID: 10543988)
J Biomed Mater Res A. 2017 Dec;105(12):3369-3383. (PMID: 28795778)
PeerJ. 2017 Jan 12;5:e2821. (PMID: 28097053)
Cells Tissues Organs. 2011;194(2-4):313-9. (PMID: 21576907)
PLoS One. 2009 Jul 03;4(7):e6131. (PMID: 19584927)
J Leukoc Biol. 2007 Mar;81(3):741-7. (PMID: 17135572)
Brain Behav Evol. 1977 Feb;14(1-2):10-22. (PMID: 13907)
J Clin Invest. 1998 Apr 1;101(7):1468-78. (PMID: 9525990)
J Bone Miner Res. 1998 Jul;13(7):1101-11. (PMID: 9661074)
Grant Information:: BB/M013545/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
Contributed Indexing:: Keywords: bone regeneration; electrical stimulation; faradic by-products; macrophages; preosteoblasts
Substance Nomenclature:: 0 (Bmp2 protein, mouse)
0 (Bone Morphogenetic Protein 2)
0 (Spp1 protein, mouse)
106441-73-0 (Osteopontin)
Entry Date(s):: Date Created: 20190821 Date Completed: 20200916 Latest Revision: 20210110
Update Code:: 20240105
PubMed Central ID:: PMC6899728
DOI:: 10.1002/bit.27142
PMID:: 31429922
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The capability of electrical stimulation (ES) in promoting bone regeneration has already been addressed in clinical studies. However, its mechanism is still being investigated and discussed. This study aims to investigate the responses of macrophages (J774A.1) and preosteoblasts (MC3T3-E1) to ES and the faradic by-products from ES. It is found that pH of the culture media was not significantly changed, whereas the average hydrogen peroxide concentration was increased by 3.6 and 5.4 µM after 1 and 2 hr of ES, respectively. The upregulation of Bmp2 and Spp1 messenger RNAs was observed after 3 days of stimulation, which is consistent among two cell types. It is also found that Spp1 expression of macrophages was partially enhanced by faradic by-products. Osteogenic differentiation of preosteoblasts was not observed during the early stage of ES as the level of Runx2 expression remains unchanged. However, cell proliferation was impaired by the excessive current density from the electrodes, and also faradic by-products in the case of macrophages. This study shows that macrophages could respond to ES and potentially contribute to the bone formation alongside preosteoblasts. The upregulation of Bmp2 and Spp1 expressions induced by ES could be one of the mechanisms behind the electrically stimulated osteogenesis.
(© 2019 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc.)

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